Paenibacillus elgii sp. nov., with broad antimicrobial activity

Publication Type:Journal Article
Year of Publication:2004
Authors:D. - S. Kim, Bae, C. - Y., Jeon, J. - J., Chun, S. - J., Oh, H. Woo, Hong, S. Gyu, Baek, K. - S., Moon, E. Young, Bae, K. Sook
Journal:Int J Syst Evol Microbiol
Date Published:Nov
Keywords:Anaerobiosis, Anti-Bacterial Agents, Bacterial Typing Techniques, Base Composition, DNA: Bacterial, DNA: Ribosomal, Fatty Acids, Genes: rRNA, Gram-Positive Endospore-Forming Bacteria, Molecular Sequence Data, Perilla frutescens, Phylogeny, Plant Roots, RNA: Bacterial, RNA: Ribosomal: 16S, Sequence Analysis: DNA, Temperature

Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol%, and the major fatty acid was anteiso-C(15 : 0) (54.1 %). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 % with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 %, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)).

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith