TY - JOUR T1 - Paenibacillus elgii sp. nov., with broad antimicrobial activity JF - Int J Syst Evol Microbiol Y1 - 2004 DO - 10.1099/ijs.0.02414-0 A1 - Dal-Soo Kim A1 - Cheol-Yong Bae A1 - Jae-Jin Jeon A1 - Sam-Jae Chun A1 - Hyun Woo Oh A1 - Soon Gyu Hong A1 - Keun-Sik Baek A1 - Eun Young Moon A1 - Kyung Sook Bae SP - 2031–5 KW - Anaerobiosis KW - Anti-Bacterial Agents KW - Bacterial Typing Techniques KW - Base Composition KW - DNA: Bacterial KW - DNA: Ribosomal KW - Fatty Acids KW - Genes: rRNA KW - Gram-Positive Endospore-Forming Bacteria KW - Molecular Sequence Data KW - Perilla frutescens KW - Phylogeny KW - Plant Roots KW - RNA: Bacterial KW - RNA: Ribosomal: 16S KW - Sequence Analysis: DNA KW - Temperature AB - Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol%, and the major fatty acid was anteiso-C(15 : 0) (54.1 %). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 % with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 %, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)). VL - 54 ER -