[Study on intraspecific genetic diversity in different plant populations of Pogostemon cabli]

Publication Type:Journal Article
Year of Publication:2006
Authors:C. -mei Pan, Li, W., He, H., Deng, W. -qiu, Li, T. -hui, Xu, H. -hua
Journal:Zhongguo Zhong Yao Za Zhi
Date Published:May
Keywords:China, Cluster Analysis, Dna Fingerprinting, DNA: Plant, Ecosystem, Lamiaceae, Phylogeny, Plants: Medicinal, Polymorphism: Genetic, Random Amplified Polymorphic DNA Technique

OBJECTIVE: To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin. METHOD: Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character. RESULT: A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species. CONCLUSION: It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith