Isolation, characterization and cross-species amplifications of microsatellite loci from Conradina (Lamiaceae)

Publication Type:Journal Article
Year of Publication:2008
Authors:C. E. Edwards, Soltis, D. E., Soltis, P. S.
Journal:Mol Ecol Resour
Date Published:Jan
Keywords:Conradina, Conservation, Hybridization, Lamiaceae, Microsatellites, Species Boundaries

We report the isolation of microsatellite loci from three species in the genus Conradina (Lamiaceae). To ensure their utility for multiple species, loci were screened for amplification and variability in all six Conradina species; 11 loci demonstrated high levels of amplification and polymorphism in most species. These 11 loci were characterized in 20 individuals from one population of Conradina brevifolia; alleles per locus ranged from five to 15, and observed heterozygosity ranged from 0.30 to 0.90. These microsatellites will be used to clarify species limits, detect interspecific hybridization, and understand the partitioning of genetic variation in each species of Conradina.

Scratchpads developed and conceived by (alphabetical): Ed Baker, Katherine Bouton Alice Heaton Dimitris Koureas, Laurence Livermore, Dave Roberts, Simon Rycroft, Ben Scott, Vince Smith