TY - JOUR T1 - [Analysis on genetic diversity of different Salvia miltiorrhiza geographical populations in China] JF - Zhongguo Zhong Yao Za Zhi Y1 - 2007 A1 - Bing Wang A1 - Yong Zhang A1 - Cheng-Bin Chen A1 - Xiu-Lan Li A1 - Rui-Yang Chen A1 - Li Chen SP - 1988–91 KW - Amplified Fragment Length Polymorphism Analysis KW - China KW - Cluster Analysis KW - DNA Primers KW - DNA: Plant KW - Genetic Variation KW - Genetics: Population KW - Phylogeny KW - Plants: Medicinal KW - Salvia miltiorrhiza AB - OBJECTIVE: To research on genetic diversity of different Salvia miltiorrhiza geographical populations in China. METHOD: The genetic diversity of 27 S. miltiorrhiza geographical populations from ten provinces in China was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE and SPSS. RESULT: The ten primers employed produced a total of 528 discernable and reproduceable amplified fragments. There were 476 polymorphic brands. The percentage of polymorphic bands with in different populations was 90.15%. Genetic diversity analysis showed that Neis gene diversity (He) was 0.261 2 and Shannon's genetic diversity index (1) was 0.403 3. The coefficient of gene similarity was 0.504 0-0.789 0 between populations. The cluster map including all samples were obtained by UPGMA. In the map, there were seven cluster groups and one individual outside the groups. CONCLUSION: The genetic diversity with in different geographical population of S. miltiorrhiza in China is plentiful. VL - 32 ER - TY - JOUR T1 - [Correlation between ITS genotype and geographical distribution of Pogostemon cablin] JF - Yao Xue Xue Bao Y1 - 2007 A1 - Ying Zhang A1 - Yao Chen A1 - Jin-Chao Zhang A1 - Meng-Su Yang A1 - Hui Cao A1 - Pei-Gen Xiao SP - 93–7 KW - Base Sequence KW - Biodiversity KW - China KW - Cluster Analysis KW - DNA: Plant KW - DNA: Ribosomal KW - DNA: Ribosomal Spacer KW - Genotype KW - Geography KW - Lamiaceae KW - Molecular Sequence Data KW - Phylogeny KW - Plant Leaves KW - Plants: Medicinal KW - Sequence Analysis: DNA AB - To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification. VL - 42 ER - TY - JOUR T1 - [Study on intraspecific genetic diversity in different plant populations of Pogostemon cabli] JF - Zhongguo Zhong Yao Za Zhi Y1 - 2006 A1 - Chao-mei Pan A1 - Wei Li A1 - Hong He A1 - Wang-qiu Deng A1 - Tai-hui Li A1 - Hong-hua Xu SP - 723–6 KW - China KW - Cluster Analysis KW - Dna Fingerprinting KW - DNA: Plant KW - Ecosystem KW - Lamiaceae KW - Phylogeny KW - Plants: Medicinal KW - Polymorphism: Genetic KW - Random Amplified Polymorphic DNA Technique AB - OBJECTIVE: To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin. METHOD: Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character. RESULT: A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species. CONCLUSION: It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm. VL - 31 ER - TY - JOUR T1 - Genetic diversity and population structure of Lamiophlomis rotata (Lamiaceae), an endemic species of Qinghai-Tibet Plateau JF - Genetica Y1 - 2006 DO - 10.1007/s10709-006-7517-y A1 - Jimei Liu A1 - Li Wang A1 - Yupeng Geng A1 - Qingbiao Wang A1 - Lijun Luo A1 - Yang Zhong SP - 385–94 KW - China KW - Conservation of Natural Resources KW - DNA: Plant KW - Genetic Variation KW - Lamiaceae KW - Minisatellite Repeats KW - Phylogeny KW - Random Amplified Polymorphic DNA Technique KW - Tibet AB - {Lamiophlomis rotata (Lamiaceae), a perennial medicinal herb, is endemic to the Qinghai-Tibet Plateau. A total of 188 individuals from eight natural populations of L. rotata in Qinghai-Tibet Plateau (four from Tibet, two from Yunnan, and two from Qinghai) were analyzed using intersimple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) techniques. Our results revealed that the level of genetic variation in L. rotata was relatively high (P = 94.85% VL - 128 ER - TY - JOUR T1 - Stachytarpheta leaf curl virus is a novel monopartite begomovirus species JF - Arch Virol Y1 - 2005 DO - 10.1007/s00705-005-0568-z A1 - Q Xiong A1 - S Fan A1 - X Guo A1 - X Zhou SP - 2257–70 KW - China KW - DNA: Viral KW - Geminiviridae KW - Lamiaceae KW - Molecular Sequence Data KW - Phylogeny KW - Plant Diseases KW - Plant Leaves KW - Polymerase Chain Reaction AB - Begomovirus isolates were obtained from Stachytarpheta jamaicensis plants showing leaf curl and chlorosis symptoms collected in the Hainan province of China. The complete sequences of isolates Hn5-4, Hn6-1, Hn30 and Hn34 were determined to be 2748, 2751, 2748 and 2748 nucleotides long, respectively. The complete sequences of the four isolates share more than 94.9% nucleotide sequence identity, but all of them have less than 86% nucleotide sequence identity with other reported begomoviruses. The molecular data show that Hn5-4, Hn6-1, Hn30 and Hn34 are isolates of a distinct begomovirus species, for which the name Stachytarpheta leaf curl virus (StaLCV) is proposed. PCR and Southern blot analyses demonstrate that all the collected field samples are not associated with DNAbeta or DNA-B components. An infectious clone of StaLCV isolate Hn5-4 was constructed, and could efficiently infect Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Lycopersicon esculentum and Petunia hybrida plants, inducing upward leaf roll and vein swelling symptoms. In addition, we illustrate that StaLCV can functionally interact with distinct DNAbeta molecules in plants. VL - 150 ER -