TY - JOUR T1 - Molecular phylogenetics of the Macaronesian-endemic genus Bystropogon (Lamiaceae): palaeo-islands, ecological shifts and interisland colonizations JF - Molecular ecology Y1 - 2005 DO - 10.1111/j.1365-294X.2005.02487.x A1 - Jennifer L Trusty A1 - Richard G Olmstead A1 - Arnoldo Santos-Guerra A1 - Susana Sá-Fontinha A1 - Javier Francisco-Ortega SP - 1177–89 KW - DNA: Chloroplast KW - DNA: Ribosomal KW - DNA: Ribosomal Spacer KW - Genetic Variation KW - Geography KW - Lamiaceae KW - Phylogeny KW - Sequence Analysis: DNA AB - Abstract A molecular phylogenetic study of Bystropogon L'Her. (Lamiaceae) is presented. We performed a cladistic analysis of nucleotide sequences of the internal transcribed spacers (ITS), of the nuclear ribosomal DNA, and of the trnL gene and trnL-trnF intergenic spacer of the chloroplast DNA. Bystropogon odoratissimus is the only species endemic to the Canary Islands that occurs in the three palaeo-islands of Tenerife. This species is not part of an early diverging lineage of Bystropogon and we suggest that it has a recent origin. This phylogenetic pattern is followed by most of the species endemic to the palaeo-islands of Tenerife. The two sections currently recognized in Bystropogon form two monophyletic groups. Taxa belonging to the section Bystropogon clade show interisland colonization limited to the Canary Islands with ecological shifts among three ecological zones. Taxa from the section Canariense clade show interisland colonization both within the Canary Islands and between the Canary Islands and Madeira. Speciation events within this clade are mostly limited to the laurel forest. The genus has followed a colonization route from the Canaries towards Madeira. This route has also been followed by at least five other plant genera with species endemic to Macaronesia. Major incongruences were found between the current infrasectional classification and the molecular phylogeny, because the varieties of Bystropogon origanifolius and Bystropogon canariensis do not form two monophyletic groups. The widespread B. origanifolius appears as progenitor of the other species in section Bystropogon with a more restricted distribution. VL - 14 ER - TY - JOUR T1 - Phylogenetic relationships between Clerodendrum (Lamiaceae) and other Ajugoid genera inferred from nuclear and chloroplast DNA sequence data JF - Molecular Phylogenetics and Evolution Y1 - 2004 DO - 10.1016/j.ympev.2003.11.011 A1 - Dorothy A Steane A1 - Rogier P J de Kok A1 - Richard G Olmstead SP - 39–45 KW - Cell Nucleus KW - Chloroplasts KW - Clerodendrum KW - Dna KW - Genes: Plant KW - Likelihood Functions KW - Phylogeny KW - RNA: Ribosomal KW - Species Specificity AB - Over the last two centuries the circumscription of the large, pan-tropical genus Clerodendrum (Lamiaceae) has changed frequently, as different authorities have added or removed taxa on the basis of various morphological characters. With the development of molecular methods for systematic research the process of circumscribing taxa has become increasingly analytical. When morphology signals the possibility that taxa are closely related, molecular methods can be used to test the hypothesis objectively. Aegiphila, Amasonia, Huxleya, and Kalaharia are similar morphologically to Clerodendrum. In this paper we use nuclear ribosomal ITS and chloroplast ndhF sequence data to clarify the positions of these four genera relative to Clerodendrum. We show that the Australian monotypic genus Huxleya evolved from within Clerodendrum. Accordingly, we sink Huxleya into Clerodendrum and make a new combination, Clerodendrum linifolium. VL - 32 ER -