01923nas a2200133   4500008004100000245011500041210006900156300001400225490000700239520138400246100001601630700001201646856013101658       1994                        eng d00aMolecular systematics of the Nepetoideae (family Labiatae): phylogenetic implications from rbcL gene sequences0 aMolecular systematics of the Nepetoideae family Labiatae phyloge  a635–6450 v493 aTotal DNA was extracted from 41 species (20 genera) of the subfamily Nepetoideae (family Labiatae). Using rbcl-specific primers, the rbcL gene was amplified by polymerase chain reaction (PCR) and sequenced directly. RbcL sequences were evaluated with character state (maximum parsimony; PAUP) and distance methods (neighbour-joining; MEGA). In agreement with classical systematics all taxa studied cluster within the Nepetoideae and are clearly distinguished from members of the subfamily Lamioideae. A number of distinctive clades are apparent within the Nepetoideae: I - Collinsonia, II - Lavandula, III - Agastache, Glechoma, IV - Satureja, Hyssopus, Dracocephalum, V - Nepeta, VI - Hormium, VII - Prunella, VIII - Melissa, Ocimum, IX - Monarda, Mentha, X - Origanum, Thymus, XI - Salvia, XII - Rosmarinus, and XIII - Perovskia. At least five main branches representing the clades I, II, III to VII, VIII, and IX to XIII respectively, can be distinguished within the Nepetoideae studied. They might be considered representing the tribes (according to Cantino, 1992) Elsholtzieae (I), Lavanduleae (II), and Mentheae (III-XIII). The tribe Mentheae needs to be subdivided into at least three main groups (clades III-VII; VIII and IX-XIII). Majorana hortensis which is often classified as Origanum hortensis does not cluster with Origanum and deserves a generic status of its own.1 aKaufmann, M1 aWink, M  uhttps://lamiaceae.myspecies.info/content/molecular-systematics-nepetoideae-family-labiatae-phylogenetic-implications-rbcl-gene