02452nas a2200313   4500008004100000245013100041210006900172260000800241300001400249490000700263520130700270653001801577653003001595653000801625653002501633653000901658653001901667653001601686653001001702653001701712653001601729653002201745653002601767653002501793653003301818100002101851700002601872856024001898       2008                        eng d00aApplication of essential oils in maize grain: Impact on Aspergillus section Flavi growth parameters and aflatoxin accumulation0 aApplication of essential oils in maize grain Impact on Aspergill  cJan  a324–3340 v253 aThe antifungal activity of Pimpinella anisum L. (anise), Peumus boldus Mol (boldus), Hedeoma multiflora Benth (mountain thyme), Syzygium aromaticum L. (clove), and Lippia turbinate var. integrifolia (griseb) (poleo) essential oils (EOs) against Aspergillus section Flavi was evaluated in sterile maize grain under different water activity (a(w)) condition (0.982, 0.955, and 0.90). The effect of EOs added to maize grains on growth rate, lag phase, and aflatoxin B-1 (AFB(1)) accumulation of Aspergillus section Flavi were evaluated at different water activity conditions. The five EOs analyzed have been shown to influence lag phase and growth rate. Their efficacy depended mainly on the essential oil concentrations and substrate water activity conditions. All EOs showed significant impact on AFB(1) accumulation. This effect was closely dependent on the water activity, concentration, and incubation periods. Important reduction of AFB(1) accumulation was observed in the majority of EO treatments at 11 days of incubation. Boldus, poleo, and mountain thyme EO completely inhibited AFB(1) at 2000 and 3000 mu g g(-1). Inhibition of AFB(1) accumulation was also observed when aflatoxigenic isolates grew with different concentration of EOs during 35 days. (C) 2007 Elsevier Ltd. All rights reserved.10aAflatoxin B-110aAspergillus Section Flavi10aB-110aChemical-Composition10aCorn10aEssential Oils10aField Maize10aFungi10aGrowing Wild10aMaize Grain10aMolecular-Biology10aOregano Essential Oil10aPlant Essential Oils10aVitro Antimicrobial Activity1 aBluma, Romina, V1 aEtcheverry, Miriam, G  uhttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFP-4PV2RX7-1&_user=2139813&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000054276&_version=1&_urlVersion=0&_userid=2139813&md5=168e578f163ed7acac18253bba58ec0f