02941nas a2200349   4500008004100000245016900041210006900210260000800279300001400287490000800301520176300309653002402072653001702096653002302113653002302136653002502159653001402184653002802198653001402226653002502240653001902265653002902284653003402313100001602347700001202363700002402375700001302399700001402412700001402426700001302440856013802453       1999                        eng d00aMolecular cloning and biochemical characterization of a novel anthocyanin 5-O-glucosyltransferase by mRNA differential display for plant forms regarding anthocyanin0 aMolecular cloning and biochemical characterization of a novel an  cMar  a7405–110 v2743 aUDP-glucose: anthocyanin 5-O-glucosyltransferase (5-GT) is responsible for the modification of anthocyanins to more stable molecules in complexes for co-pigmentation, supposedly resulting in a purple hue. The cDNA encoding 5-GT was isolated by a differential display applied to two different forms of anthocyanin production in Perilla frutescens var. crispa. Differential display was carried out for mRNA from the leaves of reddish-purple and green forms of P. frutescens, resulting in the isolation of five cDNA clones predominantly expressed in the red form. The cDNA encoded a polypeptide of 460 amino acids, exhibiting a low homology with the sequences of several glucosyltransferases including UDP-glucose: anthocyanidin 3-O-glucosyltransferase. By using this cDNA as the probe, we also isolated a homologous cDNA clone from a petal cDNA library of Verbena hybrida. To identify the biochemical function of the encoded proteins, these cDNAs were expressed in Saccharomyces cerevisiae cells. The recombinant proteins in the yeast extracts catalyzed the conversion of anthocyanidin 3-O-glucosides into the corresponding anthocyanidin 3,5-di-O-glucosides using UDP-glucose as a cofactor, indicating the identity of the cDNAs encoding 5-GT. Several biochemical properties (optimum pH, Km values, and sensitivity to inhibitors) were similar to those reported previously for 5-GTs. Southern blot analysis indicated the presence of two copies of 5-GT genes in the genome of both red and green forms of P. frutescens. The mRNA accumulation of the 5-GT gene was detected in the leaves of the red form but not in those of the green form and was induced by illumination of light, as observed for other structural genes for anthocyanin biosynthesis in P. frutescens.10aAmino Acid Sequence10aAnthocyanins10aCloning: Molecular10aDNA: Complementary10aGlucosyltransferases10aLamiaceae10aMolecular Sequence Data10aPhylogeny10aRecombinant Proteins10aRNA: Messenger10aSaccharomyces cerevisiae10aSequence Homology: Amino Acid1 aYamazaki, M1 aGong, Z1 aFukuchi-Mizutani, M1 aFukui, Y1 aTanaka, Y1 aKusumi, T1 aSaito, K  uhttps://lamiaceae.myspecies.info/content/molecular-cloning-and-biochemical-characterization-novel-anthocyanin-5-o-glucosyltransferase02290nas a2200277   4500008004100000245016300041210006900204260000800273300001300281490000800294520131300302653001501615653002301630653002301653653001501676653004001691653001401731653001401745653003901759653001701798100001501815700001201830700001501842700001701857856013801874       2005                        eng d00aCentromere-specific repetitive sequences from Torenia, a model plant for interspecific fertilization, and whole-mount FISH of its interspecific hybrid embryos0 aCentromerespecific repetitive sequences from Torenia a model pla  cJan  a228–350 v1093 aTorenia fournieri is a good model plant to study fertilization in plants because it is easy to observe the fertilization process due to the protruding nature of the embryo sac. To study the location and movement of chromosomes and their centromeres in early embryogenesis of interspecific hybrid plants, we isolated two families of centromere-specific tandem repetitive sequences from T. fournieri and T. bailonii, and named them the "TCEN-family" and "BCEN-family", respectively. Both sequences consisted of a repeat unit of 52 bp located in the pericentric and centric heterochromatins. All signals of both sequences were prominent, but their intensity varied among the chromosomes. DNA-blot hybridization indicated the presence of similar sequences of TCEN-family in T. concolor, N. caerulea, and "Summer Wave", whereas the BCEN-family was found only in T. bailonii, thus indicating the wide or specific distribution of their repetitive families observed. We also applied whole-mount FISH to the interspecific hybrid embryos by using TCEN- and BCEN-family sequences as probes. Our results suggest that whole-mount FISH with the species-specific centromere sequences as probes is an ideal method to analyze the dynamics of chromosomes and centromeres in interspecific fertilization and early embryogenesis.10aCentromere10aChromosomes: Plant10aCloning: Molecular10aDNA: Plant10aIn Situ Hybridization: Fluorescence10aLamiaceae10aPhylogeny10aRepetitive Sequences: Nucleic Acid10aReproduction1 aKikuchi, S1 aKish, M1 aShimizu, M1 aTsujimoto, H  uhttps://lamiaceae.myspecies.info/content/centromere-specific-repetitive-sequences-torenia-model-plant-interspecific-fertilization-and02388nas a2200325   4500008004100000245012900041210006900170260000800239300001400247490000800261520129300269653002401562653002301586653004101609653002301650653002101673653001501694653002501709653001401734653003601748653002801784653001401812653001601826653002501842100001601867700001401883700001401897700001701911856013401928       2000                        eng d00aCloning and expression of UDP-glucose: flavonoid 7-O-glucosyltransferase from hairy root cultures of Scutellaria baicalensis0 aCloning and expression of UDPglucose flavonoid 7Oglucosyltransfe  cMay  a1006–130 v2103 aA cDNA encoding UDP-glucose: baicalein 7-O-glucosyltransferase (UBGT) was isolated from a cDNA library from hairy root cultures of Scutellaria baicalensis Georgi probed with a partial-length cDNA clone of a UDP-glucose: flavonoid 3-O-glucosyltransferase (UFGT) from grape (Vitis vinifera L.). The heterologous probe contained a glucosyltransferase consensus amino acid sequence which was also present in the Scutellaria cDNA clones. The complete nucleotide sequence of the 1688-bp cDNA insert was determined and the deduced amino acid sequences are presented. The nucleotide sequence analysis of UBGT revealed an open reading frame encoding a polypeptide of 476 amino acids with a calculated molecular mass of 53,094 Da. The reaction product for baicalein and UDP-glucose catalyzed by recombinant UBGT in Escherichia coli was identified as authentic baicalein 7-O-glucoside using high-performance liquid chromatography and proton nuclear magnetic resonance spectroscopy. The enzyme activities of recombinant UBGT expressed in E. coli were also detected towards flavonoids such as baicalein, wogonin, apigenin, scutellarein, 7,4'-dihydroxyflavone and kaempferol, and phenolic compounds. The accumulation of UBGT mRNA in hairy roots was in response to wounding or salicylic acid treatments.10aAmino Acid Sequence10aBlotting: Northern10aChromatography: High Pressure Liquid10aCloning: Molecular10aEscherichia coli10aFlavonoids10aGlucosyltransferases10aLamiaceae10aMagnetic Resonance Spectroscopy10aMolecular Sequence Data10aPhylogeny10aPlant Roots10aRecombinant Proteins1 aHirotani, M1 aKuroda, R1 aSuzuki, H1 aYoshikawa, T  uhttps://lamiaceae.myspecies.info/content/cloning-and-expression-udp-glucose-flavonoid-7-o-glucosyltransferase-hairy-root-cultures