03028nas a2200301   4500008004100000245013500041210006900176260000800245300001300253490000800266520210300274653001802377653002102395653001402416653001802430653002202448653001302470653002702483653001202510653001402522653001902536653002402555653003102579653001002610100002602620700002302646856005702669       2007                        eng d00aPopulation genetic structure and hybridization patterns in the Mediterranean endemics Phlomis lychnitis and P. crinita (Lamiaceae)0 aPopulation genetic structure and hybridization patterns in the M  cOct  a735–460 v1003 aBACKGROUND AND AIMS: The historical influence of gene flow and genetic drift after the last glacial phase of the Quaternary Period is reflected in current levels of genetic diversity and population structure of plant species. Moreover, hybridization after secondary contact might also affect population genetic diversity and structure. An assessment was made of the genetic variation and hybrid zone structure in Iberian populations of the Mediterranean Phlomis lychnitis and P. crinita, for which phylogenetic relationships are controversial, and hybridization and introgression are common. METHODS: Allozyme variation at 13 loci was analysed in 1723 individual plants sampled from 35 natural locations of P. lychnitis, P. crinita subsp. malacitana and P. crinita subsp. crinita in southern and eastern Spain. Standard genetic diversity parameters were calculated and patterns of genetic structure in each taxon were tested to fit the equilibrium between gene flow and genetic drift. Individual multilocus genotypes were subjected to Bayesian clustering analysis to estimate hybridization and introgression rates for both geographic regions. KEY RESULTS: Contrasting patterns in the distribution of genetic variation among the three taxa were found. Phlomis lychnitis showed no significant inbreeding, low genetic differentiation among populations and no evidence of isolation by distance. Phlomis crinita subsp. malacitana and P. crinita subsp. crinita showed high levels of genetic structure consistent with a pattern of gene flow-drift equilibrium. Higher instances of hybridization and introgression were detected in locations from southern Spain compared with locations from eastern Spain, matching unimodal and bimodal hybrid zones, respectively. CONCLUSIONS: High instances of historical gene flow, range expansion and altitudinal movement during the Quaternary Period, and lineage sorting can explain the diversity of patterns observed. The results suggest that P. lychnitis is the most differentiated lineage in the group; however, the relationship between the three taxa remains unclear.10aBayes Theorem10aCluster Analysis10aGene Flow10aGenetic Drift10aGenetic Variation10aGenotype10aHybridization: Genetic10aPhlomis10aPhylogeny10aPlant Proteins10aPopulation Dynamics10aSequence Analysis: Protein10aSpain1 aAlbaladejo, Rafael, G1 aAparicio, Abelardo  uhttp://aob.oxfordjournals.org/content/100/4/735.long03043nas a2200337   4500008004100000245015400041210006900195260000800264300000600272490000600278520194000284653001202224653002402236653002302260653002302283653001802306653002002324653001002344653001402354653001002368653002102378653001402399653002602413653002402439653002302463100002102486700001702507700002602524700002102550856013402571       2005                        eng d00aPopulation dynamics of the Teak defoliator (Hyblaea puera Cramer) in Nilambur teak plantations using Randomly Amplified Gene Encoding Primers (RAGEP)0 aPopulation dynamics of the Teak defoliator Hyblaea puera Cramer   cJan  a10 v53 aBACKGROUND: The Teak defoliator (Hyblaea puera) is a pest moth of teak woodlands in India and other tropical regions (e.g. Thailand) and is of major economic significance. This pest is of major concern as it is involved in complete defoliation of trees during the early part of the growing season. Defoliation does not kill teak trees, but it results in huge amount of timber loss. Teak defoliator outbreaks are a regular annual feature in most teak plantations in India and it is extremely difficult to predict the exact time and place of occurrence of these outbreaks. Evidence from the study of the population dynamics of H. puera indicated habitual, short range movements of emerging moth populations, suggesting that these populations have spread to larger areas, generation after generation, affecting the entire teak plantations. We were therefore interested in investigating the temporal and spatial relationship among various population groups in Nilambur, Kerala (India) and address the cause of outbreak at the landscape level. RESULTS: The populations were classified into 'endemic', 'epicenter' and 'epidemic' populations based on the time of occurrence and size of infestation. We devised a novel method of screening nuclear and mitochondrial DNA polymorphisms using Randomly Amplified Gene Encoding Primers (RAGEP). We have used this method extensively to evaluate the species specificity, reproducibility and to discriminate among the three different characterised populations of teak defoliator. CONCLUSIONS: This method also allowed us to comment with some certainty that the endemic teak defoliator, H. puera do not play a major role in contributing to large-scale infestations. With respect to the hypotheses put forward regarding the origin of outbreaks of the moth, this study confirms the role of migration in outbreak causation, while negating the belief that endemic populations aggregate to cause an epidemic.10aAnimals10aCrops: Agricultural10aDna Fingerprinting10aDNA: Mitochondrial10aGenes: Insect10aGenetic Markers10aIndia10aLamiaceae10aMoths10aNuclear Proteins10aPhylogeny10aPolymorphism: Genetic10aPopulation Dynamics10aRepressor Proteins1 aChandrasekhar, N1 aSajeev, T, V1 aSudheendrakumar, V, V1 aBanerjee, Moinak  uhttps://lamiaceae.myspecies.info/content/population-dynamics-teak-defoliator-hyblaea-puera-cramer-nilambur-teak-plantations-using