@article {Trusty:2005p8008,
	title = {Molecular phylogenetics of the Macaronesian-endemic genus Bystropogon (Lamiaceae): palaeo-islands, ecological shifts and interisland colonizations},
	journal = {Molecular ecology},
	volume = {14},
	number = {4},
	year = {2005},
	month = {Apr},
	pages = {1177{\textendash}89},
	abstract = {Abstract A molecular phylogenetic study of Bystropogon L{\textquoteright}Her. (Lamiaceae) is presented. We performed a cladistic analysis of nucleotide sequences of the internal transcribed spacers (ITS), of the nuclear ribosomal DNA, and of the trnL gene and trnL-trnF intergenic spacer of the chloroplast DNA. Bystropogon odoratissimus is the only species endemic to the Canary Islands that occurs in the three palaeo-islands of Tenerife. This species is not part of an early diverging lineage of Bystropogon and we suggest that it has a recent origin. This phylogenetic pattern is followed by most of the species endemic to the palaeo-islands of Tenerife. The two sections currently recognized in Bystropogon form two monophyletic groups. Taxa belonging to the section Bystropogon clade show interisland colonization limited to the Canary Islands with ecological shifts among three ecological zones. Taxa from the section Canariense clade show interisland colonization both within the Canary Islands and between the Canary Islands and Madeira. Speciation events within this clade are mostly limited to the laurel forest. The genus has followed a colonization route from the Canaries towards Madeira. This route has also been followed by at least five other plant genera with species endemic to Macaronesia. Major incongruences were found between the current infrasectional classification and the molecular phylogeny, because the varieties of Bystropogon origanifolius and Bystropogon canariensis do not form two monophyletic groups. The widespread B. origanifolius appears as progenitor of the other species in section Bystropogon with a more restricted distribution.},
	keywords = {DNA: Chloroplast, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Geography, Lamiaceae, Phylogeny, Sequence Analysis: DNA},
	doi = {10.1111/j.1365-294X.2005.02487.x},
	author = {Jennifer L Trusty and Richard G Olmstead and Arnoldo Santos-Guerra and Susana S{\'a}-Fontinha and Javier Francisco-Ortega}
}
@article {Kikuchi:2005p8019,
	title = {Centromere-specific repetitive sequences from Torenia, a model plant for interspecific fertilization, and whole-mount FISH of its interspecific hybrid embryos},
	journal = {Cytogenet Genome Res},
	volume = {109},
	number = {1-3},
	year = {2005},
	month = {Jan},
	pages = {228{\textendash}35},
	abstract = {Torenia fournieri is a good model plant to study fertilization in plants because it is easy to observe the fertilization process due to the protruding nature of the embryo sac. To study the location and movement of chromosomes and their centromeres in early embryogenesis of interspecific hybrid plants, we isolated two families of centromere-specific tandem repetitive sequences from T. fournieri and T. bailonii, and named them the "TCEN-family" and "BCEN-family", respectively. Both sequences consisted of a repeat unit of 52 bp located in the pericentric and centric heterochromatins. All signals of both sequences were prominent, but their intensity varied among the chromosomes. DNA-blot hybridization indicated the presence of similar sequences of TCEN-family in T. concolor, N. caerulea, and "Summer Wave", whereas the BCEN-family was found only in T. bailonii, thus indicating the wide or specific distribution of their repetitive families observed. We also applied whole-mount FISH to the interspecific hybrid embryos by using TCEN- and BCEN-family sequences as probes. Our results suggest that whole-mount FISH with the species-specific centromere sequences as probes is an ideal method to analyze the dynamics of chromosomes and centromeres in interspecific fertilization and early embryogenesis.},
	keywords = {Centromere, Chromosomes: Plant, Cloning: Molecular, DNA: Plant, In Situ Hybridization: Fluorescence, Lamiaceae, Phylogeny, Repetitive Sequences: Nucleic Acid, Reproduction},
	doi = {10.1159/000082405},
	author = {S Kikuchi and M Kishii and M Shimizu and H Tsujimoto}
}
@article {Brauchler:2005p1116,
	title = {Polyphyly of the genus Micromeria (Lamiaceae) - evidence from cpDNA sequence data},
	journal = {Taxon},
	volume = {54},
	number = {3},
	year = {2005},
	month = {Jan},
	pages = {639{\textendash}650},
	abstract = {Micromeria Benth. (Lamiaceae, Nepetoideac) is a very common genus in the Mediterranean region. To test the monophyly of the genus and to elucidate its phylogenetic placement within subtribe Menthinae (Dumort) Endl, of tribe Mentheae Dumort we performed parsimony analysis of trnK intron sequence data of 51 accessions representing 15 genera of Nepetoideae and two genera of subfamily Ajugoideae. Tree topology reveals a well-supported "core group" indicating four distinct lineages. The first one comprises three species of Satureja L. s.str., the second one includes taxa of the genus Clinopodium L. from both the Old and the New World, paraphyletic with respect to Monarda L. and two species of Micromeria section Pseudomelissa Benth. A third group contains all samples of the remaining Micromeria species. Within this monophyly, a western lineage including taxa, from NW Africa, the Balearic, and the Canary Islands, is sister to an eastern lineage with species distributed from SE Asia to the western Mediterranean. In a further clade the genera Thymbra L., Thymus L., and Origanum L. are grouped together. Combined analysis using a reduced dataset of trnK/trnL-F sequences increased support for the infrageneric resolution within Micromeria. Based on the phylogenetic reconstructions there is evidence that the genus as currently circumscribed is polyphyletic. Results are discussed in the context of morphology, karyology, and biogeography, outlining the necessity of removing section Pseudomelissa from Micromeria.},
	keywords = {Clinopodium, Dna-Sequences, Genera, Labiatae, Lamiaceae, Micromeria, Molecular phylogeny, Polyphyly, Trnk, trnL-F},
	author = {C Brauchler and H Meimberg and T Abele and G Heubl}
}
@article {Chandrasekhar:2005p8011,
	title = {Population dynamics of the Teak defoliator (Hyblaea puera Cramer) in Nilambur teak plantations using Randomly Amplified Gene Encoding Primers (RAGEP)},
	journal = {BMC Ecol},
	volume = {5},
	year = {2005},
	month = {Jan},
	pages = {1},
	abstract = {BACKGROUND: The Teak defoliator (Hyblaea puera) is a pest moth of teak woodlands in India and other tropical regions (e.g. Thailand) and is of major economic significance. This pest is of major concern as it is involved in complete defoliation of trees during the early part of the growing season. Defoliation does not kill teak trees, but it results in huge amount of timber loss. Teak defoliator outbreaks are a regular annual feature in most teak plantations in India and it is extremely difficult to predict the exact time and place of occurrence of these outbreaks. Evidence from the study of the population dynamics of H. puera indicated habitual, short range movements of emerging moth populations, suggesting that these populations have spread to larger areas, generation after generation, affecting the entire teak plantations. We were therefore interested in investigating the temporal and spatial relationship among various population groups in Nilambur, Kerala (India) and address the cause of outbreak at the landscape level. RESULTS: The populations were classified into {\textquoteright}endemic{\textquoteright}, {\textquoteright}epicenter{\textquoteright} and {\textquoteright}epidemic{\textquoteright} populations based on the time of occurrence and size of infestation. We devised a novel method of screening nuclear and mitochondrial DNA polymorphisms using Randomly Amplified Gene Encoding Primers (RAGEP). We have used this method extensively to evaluate the species specificity, reproducibility and to discriminate among the three different characterised populations of teak defoliator. CONCLUSIONS: This method also allowed us to comment with some certainty that the endemic teak defoliator, H. puera do not play a major role in contributing to large-scale infestations. With respect to the hypotheses put forward regarding the origin of outbreaks of the moth, this study confirms the role of migration in outbreak causation, while negating the belief that endemic populations aggregate to cause an epidemic.},
	keywords = {Animals, Crops: Agricultural, Dna Fingerprinting, DNA: Mitochondrial, Genes: Insect, Genetic Markers, India, Lamiaceae, Moths, Nuclear Proteins, Phylogeny, Polymorphism: Genetic, Population Dynamics, Repressor Proteins},
	doi = {10.1186/1472-6785-5-1},
	author = {N Chandrasekhar and T V Sajeev and V V Sudheendrakumar and Moinak Banerjee}
}
@article {Xiong:2005p8007,
	title = {Stachytarpheta leaf curl virus is a novel monopartite begomovirus species},
	journal = {Arch Virol},
	volume = {150},
	number = {11},
	year = {2005},
	month = {Nov},
	pages = {2257{\textendash}70},
	abstract = {Begomovirus isolates were obtained from Stachytarpheta jamaicensis plants showing leaf curl and chlorosis symptoms collected in the Hainan province of China. The complete sequences of isolates Hn5-4, Hn6-1, Hn30 and Hn34 were determined to be 2748, 2751, 2748 and 2748 nucleotides long, respectively. The complete sequences of the four isolates share more than 94.9\% nucleotide sequence identity, but all of them have less than 86\% nucleotide sequence identity with other reported begomoviruses. The molecular data show that Hn5-4, Hn6-1, Hn30 and Hn34 are isolates of a distinct begomovirus species, for which the name Stachytarpheta leaf curl virus (StaLCV) is proposed. PCR and Southern blot analyses demonstrate that all the collected field samples are not associated with DNAbeta or DNA-B components. An infectious clone of StaLCV isolate Hn5-4 was constructed, and could efficiently infect Nicotiana benthamiana, N. tabacum Samsun, N. glutinosa, Lycopersicon esculentum and Petunia hybrida plants, inducing upward leaf roll and vein swelling symptoms. In addition, we illustrate that StaLCV can functionally interact with distinct DNAbeta molecules in plants.},
	keywords = {China, DNA: Viral, Geminiviridae, Lamiaceae, Molecular Sequence Data, Phylogeny, Plant Diseases, Plant Leaves, Polymerase Chain Reaction},
	doi = {10.1007/s00705-005-0568-z},
	author = {Q Xiong and S Fan and X Guo and X Zhou}
}
