@article {Trusty:2005p8008,
	title = {Molecular phylogenetics of the Macaronesian-endemic genus Bystropogon (Lamiaceae): palaeo-islands, ecological shifts and interisland colonizations},
	journal = {Molecular ecology},
	volume = {14},
	number = {4},
	year = {2005},
	month = {Apr},
	pages = {1177{\textendash}89},
	abstract = {Abstract A molecular phylogenetic study of Bystropogon L{\textquoteright}Her. (Lamiaceae) is presented. We performed a cladistic analysis of nucleotide sequences of the internal transcribed spacers (ITS), of the nuclear ribosomal DNA, and of the trnL gene and trnL-trnF intergenic spacer of the chloroplast DNA. Bystropogon odoratissimus is the only species endemic to the Canary Islands that occurs in the three palaeo-islands of Tenerife. This species is not part of an early diverging lineage of Bystropogon and we suggest that it has a recent origin. This phylogenetic pattern is followed by most of the species endemic to the palaeo-islands of Tenerife. The two sections currently recognized in Bystropogon form two monophyletic groups. Taxa belonging to the section Bystropogon clade show interisland colonization limited to the Canary Islands with ecological shifts among three ecological zones. Taxa from the section Canariense clade show interisland colonization both within the Canary Islands and between the Canary Islands and Madeira. Speciation events within this clade are mostly limited to the laurel forest. The genus has followed a colonization route from the Canaries towards Madeira. This route has also been followed by at least five other plant genera with species endemic to Macaronesia. Major incongruences were found between the current infrasectional classification and the molecular phylogeny, because the varieties of Bystropogon origanifolius and Bystropogon canariensis do not form two monophyletic groups. The widespread B. origanifolius appears as progenitor of the other species in section Bystropogon with a more restricted distribution.},
	keywords = {DNA: Chloroplast, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Geography, Lamiaceae, Phylogeny, Sequence Analysis: DNA},
	doi = {10.1111/j.1365-294X.2005.02487.x},
	author = {Jennifer L Trusty and Richard G Olmstead and Arnoldo Santos-Guerra and Susana S{\'a}-Fontinha and Javier Francisco-Ortega}
}
@article {Wang:2007p7983,
	title = {[Analysis of ITS sequences of some medicinal plants and their related species in Salvia]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {12},
	year = {2007},
	month = {Dec},
	pages = {1309{\textendash}13},
	abstract = {Molecular systematic techniques were applied to reveal the genetic diversity of medicinal plants and their related species in Salvia. The internal transcribed spacer (ITS) as well as 5.8S rDNA sequences of 27 samples of Salvia were amplified using PCR method and sequenced. Mega 3.1 was used to analyze the genetic diversity within genus. The complete sequences of ITS plus 5.8S rDNA are about 612-617 bp. A phylogenetic tree generated by Neighbor-Joining method partly supported the morphological classification within Salvia, but incompatible results were also obtained in the treatment of phylogenetic positions of some species such as Salvia trijuga, Salvia flava var. flava and Salvia flava var. megalentha. The ITS regions of present Salria species showed considerable variation between subgenera in contrast with the conservative 5.8S rDNA sequences. The native Salvia species might have a different origin from the foreign species. The phylogenetic positions of subgenera and sections inferred by ITS analysis were comparable with that of traditional classification, while the phylogeny within sections is still doubtful due to limited information in ITS sequence and need to be further proved by other evidence. ITS analysis in this study supports the rationality of using species from Drymosphace section as substitute drug resources of Dan shen, but also reveals significant genetic differences between high mountain Dan shen species such as Salvia przewalskii with traditional Dan shen origins.},
	keywords = {Base Sequence, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Phylogeny, Plants: Medicinal, Polymerase Chain Reaction, RNA: Ribosomal: 5.8S, Salvia, Sequence Alignment, Sequence Analysis: DNA},
	author = {Ying Wang and Da-hui Li and Ying-tao Zhang}
}
@article {Zhang:2007p7990,
	title = {[Correlation between ITS genotype and geographical distribution of Pogostemon cablin]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {1},
	year = {2007},
	month = {Jan},
	pages = {93{\textendash}7},
	abstract = {To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.},
	keywords = {Base Sequence, Biodiversity, China, Cluster Analysis, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genotype, Geography, Lamiaceae, Molecular Sequence Data, Phylogeny, Plant Leaves, Plants: Medicinal, Sequence Analysis: DNA},
	author = {Ying Zhang and Yao Chen and Jin-Chao Zhang and Meng-Su Yang and Hui Cao and Pei-Gen Xiao}
}
@article {Stukenbrock:2005p8014,
	title = {Distribution of dominant arbuscular mycorrhizal fungi among five plant species in undisturbed vegetation of a coastal grassland},
	journal = {Mycorrhiza},
	volume = {15},
	number = {7},
	year = {2005},
	month = {Nov},
	pages = {497{\textendash}503},
	abstract = {Most plant species in mixed grassland vegetation are colonized by arbuscular mycorrhizal (AM) fungi. Previous studies have reported differences in host preferences among AM fungi, although the fungi are known to lack host specificity. In the present study, the distribution of phylogenetic groups of AM fungi belonging to a clade of Glomus species was studied in five plant species from a coastal grassland in Denmark. The occurrence of the fungi was determined by PCR analyses of fungal large subunit ribosomal DNA sequences amplified from root fragments using a specific primer set. The results showed that the dominant Glomus species were able to colonize all the studied plant species, supporting the view that the AM fungi represent a large underground interconnecting mycelial network.},
	keywords = {Angiosperms, Asteraceae, Denmark, DNA: Fungal, DNA: Ribosomal, Ecosystem, Fungi, Mycorrhizae, Phylogeny, Plant Roots, Polymerase Chain Reaction, Sequence Analysis: DNA, Sequence Homology, Thymus Plant},
	doi = {10.1007/s00572-005-0357-2},
	author = {Eva H Stukenbrock and S{\o}ren Rosendahl}
}
@article {Yang:2006p8002,
	title = {[Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species]},
	journal = {Nan Fang Yi Ke Da Xue Xue Bao},
	volume = {26},
	number = {11},
	year = {2006},
	month = {Nov},
	pages = {1593{\textendash}5},
	abstract = {OBJECTIVE: To analyze the nuclear ribosome DNA (nrDNA) internal transcribed spacer (ITS) sequences of 4 Leonurus species, and the possibility of using them for molecular authentication of the crude drugs from the genus. METHODS: The nrDNA ITS sequence (including ITS1, 5.8S rDNA, ITS2, and partial 18S rDNA and 26S rDNA) of L. japonicus and its 3 adulterant species were amplified and sequenced, and CLUSTRAL X and MEGA software was employed for analysis. RESULTS: The variation of ITS1 and ITS2 between L. japonicus and its adulterant species ranged between 7.2\% and 18.8\% and between 14.2\% and 27\%, respectively. The phylogenic tree derived from the dendrograms based on the ITS sequence data contained some discrepancy from the traditional classification. CONCLUSION: The nrDNA ITS sequences can be used potentially as efficient markers for identification of L. japonicus and its adulterants, and further study is needed for studying the phylogeny of Leonurus.},
	keywords = {DNA: Plant, DNA: Ribosomal Spacer, Leonurus, Molecular Sequence Data, Phylogeny, Sequence Analysis: DNA, Species Specificity},
	author = {Zhi-Ye Yang and Zhi Chao and Ke-Ke Huo and Bing-Yi Wu and Sheng-li Pan}
}
@article {TapernouxLuthi:2004p8016,
	title = {Cloning, functional expression, and characterization of the raffinose oligosaccharide chain elongation enzyme, galactan:galactan galactosyltransferase, from common bugle leaves},
	journal = {Plant Physiol},
	volume = {134},
	number = {4},
	year = {2004},
	month = {Apr},
	pages = {1377{\textendash}87},
	abstract = {Galactan:galactan galactosyltransferase (GGT) is a unique enzyme of the raffinose family oligosaccharide (RFO) biosynthetic pathway. It catalyzes the chain elongation of RFOs without using galactinol (alpha-galactosyl-myoinositol) by simply transferring a terminal alpha-galactosyl residue from one RFO molecule to another one. Here, we report the cloning and functional expression of a cDNA encoding GGT from leaves of the common bugle (Ajuga reptans), a winter-hardy long-chain RFO-storing Lamiaceae. The cDNA comprises an open reading frame of 1215 bp. Expression in tobacco (Nicotiana plumbaginifolia) protoplasts resulted in a functional recombinant protein, which showed GGT activity like the previously described purified, native GGT enzyme. At the amino acid level, GGT shows high homologies (>60\%) to acid plant alpha-galactosidases of the family 27 of glycosylhydrolases. It is clearly distinct from the family 36 of glycosylhydrolases, which harbor galactinol-dependent raffinose and stachyose synthases as well as alkaline alpha-galactosidases. Physiological studies on the role of GGT confirmed that GGT plays a key role in RFO chain elongation and carbon storage. When excised leaves were exposed to chilling temperatures, levels of GGT transcripts, enzyme activities, and long-chain RFO concentrations increased concomitantly. On a whole-plant level, chilling temperatures induced GGT expression mainly in the roots and fully developed leaves, both known RFO storage organs of the common bugle, indicating an adaptation of the metabolism from active growth to transient storage in the cold.},
	keywords = {Ajuga, Amino Acid Sequence, Cloning: Molecular, Cold Temperature, DNA: Complementary, Galactosyltransferases, Gene Expression Regulation: Enzymologic, Gene Expression Regulation: Plant, Molecular Sequence Data, Phylogeny, Plant Leaves, Plant Proteins, Raffinose, Sequence Analysis: DNA, Sequence Homology: Amino Acid, Tobacco},
	doi = {10.1104/pp.103.036210},
	author = {Esther M Tapernoux-L{\"u}thi and Andreas B{\"o}hm and Felix Keller}
}
@article {Meimberg:2006p7985,
	title = {Molecular evidence for adaptive radiation of Micromeria Benth. (Lamiaceae) on the Canary Islands as inferred from chloroplast and nuclear DNA sequences and ISSR fingerprint data},
	journal = {Molecular Phylogenetics and Evolution},
	volume = {41},
	number = {3},
	year = {2006},
	month = {Dec},
	pages = {566{\textendash}78},
	abstract = {The Canary Islands have been a focus for phylogeographic studies on the colonization and diversification of endemic angiosperm taxa. Based on phylogeographic patterns, both inter island colonization and adaptive radiation seem to be the driving forces for speciation in most taxa. Here, we investigated the diversification of Micromeria on the Canary Islands and Madeira at the inter- and infraspecific level using inter simple sequence repeat PCR (ISSR), the trnK-Intron and the trnT-trnL-spacer of the cpDNA and a low copy nuclear gene. The genus Micromeria (Lamiaceae, Mentheae) includes 16 species and 13 subspecies in Macaronesia. Most taxa are restricted endemics, or grow in similar ecological conditions on two islands. An exception is M. varia, a widespread species inhabits the lowland scrub on each island of the archipelago and could represent an ancestral taxon from which radiation started on the different islands. Our analyses support a split between the "eastern" islands Fuerteventura, Lanzarote and Gran Canaria and the "western" islands Tenerife, La Palma and El Hierro. The colonization of Madeira started from the western Islands, probably from Tenerife as indicated by the sequence data. We identified two lineages of Micromeria on Gomera but all other islands appear to be colonized by a single lineage, supporting adaptive radiation as the major evolutionary force for the diversification of Micromeria. We also discuss the possible role of gene flow between lineages of different Micromeria species on one island after multiple colonizations.},
	keywords = {Adaptation: Biological, Atlantic Islands, Dna Fingerprinting, DNA Primers, DNA: Chloroplast, Genetic Variation, Geranyltranstransferase, Introns, Lamiaceae, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Analysis: DNA},
	doi = {10.1016/j.ympev.2006.05.037},
	author = {Harald Meimberg and Tilmann Abele and Christian Br{\"a}uchler and John K McKay and Pedro L P{\'e}rez de Paz and G{\"u}nther Heubl}
}
@article {Kim:2004p8024,
	title = {Paenibacillus elgii sp. nov., with broad antimicrobial activity},
	journal = {Int J Syst Evol Microbiol},
	volume = {54},
	number = {Pt 6},
	year = {2004},
	month = {Nov},
	pages = {2031{\textendash}5},
	abstract = {Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol\%, and the major fatty acid was anteiso-C(15 : 0) (54.1 \%). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 \% with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 \%, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)).},
	keywords = {Anaerobiosis, Anti-Bacterial Agents, Bacterial Typing Techniques, Base Composition, DNA: Bacterial, DNA: Ribosomal, Fatty Acids, Genes: rRNA, Gram-Positive Endospore-Forming Bacteria, Molecular Sequence Data, Perilla frutescens, Phylogeny, Plant Roots, RNA: Bacterial, RNA: Ribosomal: 16S, Sequence Analysis: DNA, Temperature},
	doi = {10.1099/ijs.0.02414-0},
	author = {Dal-Soo Kim and Cheol-Yong Bae and Jae-Jin Jeon and Sam-Jae Chun and Hyun Woo Oh and Soon Gyu Hong and Keun-Sik Baek and Eun Young Moon and Kyung Sook Bae}
}
@article {Tzanetakis:2007p7987,
	title = {Identification, detection and transmission of a new vitivirus from Mentha},
	journal = {Arch Virol},
	volume = {152},
	number = {11},
	year = {2007},
	month = {Jan},
	pages = {2027{\textendash}33},
	abstract = {Mentha x gracilis {\textquoteright}Variegata{\textquoteright} is an ornamental clone with a phenotype caused by virus infection. Several clones were ordered from mail-order nurseries in an attempt to identify a virus consistently associated with symptoms. One of these clones did not exhibit typical {\textquoteright}Variegata{\textquoteright} symptoms, and steps were taken to identify any agents causing the {\textquoteright}off-type{\textquoteright} symptoms. One of the viruses identified in the atypical {\textquoteright}Variegata{\textquoteright} clone is a previously unknown virus, a member of the family Flexiviridae. Sequence and phylogenetic analysis indicate that the virus, designated as mint virus-2, is related to members of the species Grapevine virus A, Grapevine virus B and Heracleum latent virus, placing it in the genus Vitivirus. A detection protocol for the virus has been developed, and the mint aphid (Ovatus crataegarius) was able to transmit the virus in the presence of a helper virus but not from single infected plants.},
	keywords = {Animals, Aphids, DNA Primers, Flexiviridae, Mentha, Molecular Sequence Data, Phylogeny, Plant Diseases, Plant Leaves, Polymerase Chain Reaction, Sequence Analysis: DNA},
	doi = {10.1007/s00705-007-1030-1},
	author = {I E Tzanetakis and J D Postman and R R Martin}
}
