@article {Wang:2007p7984,
	title = {[Analysis on genetic diversity of different Salvia miltiorrhiza geographical populations in China]},
	journal = {Zhongguo Zhong Yao Za Zhi},
	volume = {32},
	number = {19},
	year = {2007},
	month = {Oct},
	pages = {1988{\textendash}91},
	abstract = {OBJECTIVE: To research on genetic diversity of different Salvia miltiorrhiza geographical populations in China. METHOD: The genetic diversity of 27 S. miltiorrhiza geographical populations from ten provinces in China was estimated using amplified fragment length polymorphism (AFLP) markers. The data of amplified bands were analyzed by the software POPGENE and SPSS. RESULT: The ten primers employed produced a total of 528 discernable and reproduceable amplified fragments. There were 476 polymorphic brands. The percentage of polymorphic bands with in different populations was 90.15\%. Genetic diversity analysis showed that Neis gene diversity (He) was 0.261 2 and Shannon{\textquoteright}s genetic diversity index (1) was 0.403 3. The coefficient of gene similarity was 0.504 0-0.789 0 between populations. The cluster map including all samples were obtained by UPGMA. In the map, there were seven cluster groups and one individual outside the groups. CONCLUSION: The genetic diversity with in different geographical population of S. miltiorrhiza in China is plentiful.},
	keywords = {Amplified Fragment Length Polymorphism Analysis, China, Cluster Analysis, DNA Primers, DNA: Plant, Genetic Variation, Genetics: Population, Phylogeny, Plants: Medicinal, Salvia miltiorrhiza},
	author = {Bing Wang and Yong Zhang and Cheng-Bin Chen and Xiu-Lan Li and Rui-Yang Chen and Li Chen}
}
@article {Wang:2007p7983,
	title = {[Analysis of ITS sequences of some medicinal plants and their related species in Salvia]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {12},
	year = {2007},
	month = {Dec},
	pages = {1309{\textendash}13},
	abstract = {Molecular systematic techniques were applied to reveal the genetic diversity of medicinal plants and their related species in Salvia. The internal transcribed spacer (ITS) as well as 5.8S rDNA sequences of 27 samples of Salvia were amplified using PCR method and sequenced. Mega 3.1 was used to analyze the genetic diversity within genus. The complete sequences of ITS plus 5.8S rDNA are about 612-617 bp. A phylogenetic tree generated by Neighbor-Joining method partly supported the morphological classification within Salvia, but incompatible results were also obtained in the treatment of phylogenetic positions of some species such as Salvia trijuga, Salvia flava var. flava and Salvia flava var. megalentha. The ITS regions of present Salria species showed considerable variation between subgenera in contrast with the conservative 5.8S rDNA sequences. The native Salvia species might have a different origin from the foreign species. The phylogenetic positions of subgenera and sections inferred by ITS analysis were comparable with that of traditional classification, while the phylogeny within sections is still doubtful due to limited information in ITS sequence and need to be further proved by other evidence. ITS analysis in this study supports the rationality of using species from Drymosphace section as substitute drug resources of Dan shen, but also reveals significant genetic differences between high mountain Dan shen species such as Salvia przewalskii with traditional Dan shen origins.},
	keywords = {Base Sequence, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Phylogeny, Plants: Medicinal, Polymerase Chain Reaction, RNA: Ribosomal: 5.8S, Salvia, Sequence Alignment, Sequence Analysis: DNA},
	author = {Ying Wang and Da-hui Li and Ying-tao Zhang}
}
@article {Zhang:2007p7990,
	title = {[Correlation between ITS genotype and geographical distribution of Pogostemon cablin]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {1},
	year = {2007},
	month = {Jan},
	pages = {93{\textendash}7},
	abstract = {To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.},
	keywords = {Base Sequence, Biodiversity, China, Cluster Analysis, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genotype, Geography, Lamiaceae, Molecular Sequence Data, Phylogeny, Plant Leaves, Plants: Medicinal, Sequence Analysis: DNA},
	author = {Ying Zhang and Yao Chen and Jin-Chao Zhang and Meng-Su Yang and Hui Cao and Pei-Gen Xiao}
}
@article {PassinhoSoares:2006p7996,
	title = {Authentication of medicinal plant botanical identity by amplified fragmented length polymorphism dominant DNA marker: inferences from the Plectranthus genus},
	journal = {Planta Med},
	volume = {72},
	number = {10},
	year = {2006},
	month = {Aug},
	pages = {929{\textendash}31},
	abstract = {In Brazil, Plectranthus species are known as "boldo" and have been used in popular medicine for analgesic and dyspeptic purposes. Plectranthus need to be well identified in order to be used as commercially genuine medicinal plants. Here we describe AFLP DNA patterns able to distinguish among different Pectranthus species. The genetic variability of P. grandis Cramer, P. barbatus Andr. and P. ornatus Codd was analyzed with two sets of AFLP primers allowing detection of 241 loci. A total of 22 monomorphic loci were identified in P. barbatus, 15 in P. grandis and 30 in P. ornatus. Among these, 5 loci were informative and species-specific to P. barbatus, 3 to P. grandis and 2 loci were unique to P. ornatus. The AFLP pattern analyzed by different clustering methods assembled individuals according to their species. So far, AFLP represents a genuine and strong method to certify medicinal plant materials.},
	keywords = {DNA: Plant, Genetic Markers, Phylogeny, Plants: Medicinal, Plectranthus, Polymerase Chain Reaction, Polymorphism: Genetic, Species Specificity},
	doi = {10.1055/s-2006-946673},
	author = {Helna Passinho-Soares and Durvalina Felix and Maria Auxiliadora Kaplan and Marcia Margis-Pinheiro and Rog{\'e}rio Margis}
}
@article {Pan:2006p8001,
	title = {[Study on intraspecific genetic diversity in different plant populations of Pogostemon cabli]},
	journal = {Zhongguo Zhong Yao Za Zhi},
	volume = {31},
	number = {9},
	year = {2006},
	month = {May},
	pages = {723{\textendash}6},
	abstract = {OBJECTIVE: To study the genetic polymorphism and intraspecific genetic differentiation of different populations of Pogostemon cablin, and find out the effective method to distinguish DNA fingerprint of different populations of P. cablin. METHOD: Five plant populations of P. cablin were analyzed by RAPD markers. PopGen 32 software for clustering analysis and calculating. Fourteen of the 80 random primers were tested to possess the stronger detecting effect of polymorphous character. RESULT: A total of 84 bands was amplified by the 10 primers, among them 17 bands were monomorphic. 67 of them were polymorphic. The results indicated that the genetic variations existed within the different plant populations of the same species. CONCLUSION: It is feasible by RAPD technique with specifically primer to analyze the genetic diversity and identify 5 plant populations of P. cablin. RAPD technique has provided a new path for identification and classification of P. cablin genetic germplasm.},
	keywords = {China, Cluster Analysis, Dna Fingerprinting, DNA: Plant, Ecosystem, Lamiaceae, Phylogeny, Plants: Medicinal, Polymorphism: Genetic, Random Amplified Polymorphic DNA Technique},
	author = {Chao-mei Pan and Wei Li and Hong He and Wang-qiu Deng and Tai-hui Li and Hong-hua Xu}
}
@article {Zhang:2003p8032,
	title = {[Application of hierarchical cluster with high-performance liquid chromatography in the classification of genus Clinopodium chinensis]},
	journal = {Zhongguo Zhong Yao Za Zhi},
	volume = {28},
	number = {9},
	year = {2003},
	month = {Sep},
	pages = {812{\textendash}6},
	abstract = {OBJECTIVE: To realize the relationships of the plants of genus C. chinensis in our country and confirm the feasible quality of the application of Hierarchical cluster in the classification of genus C. chinensis. METHOD: The chemical information of 20 samples of 10 kinds of plants was obtained with high-performance liquid chromatographic method and classification based on their chemical information was made through Hierarchical cluster. Flavonoids and saponins were the main components of the C. chinensis plants. We did the high-performance liquid chromatography from the flavonoids and saponins side respectively. RESULT: Expected results were achieved in the Hierarchical clusters of the chromatographic data of the plants of genus C. chinensis which supplied the foundation for studying crude drugs categorys and those close of C. chinensis. CONCLUSION: The cluster and identification results show the method is acceptable, simple, rapid, inexpensive and repeatable.},
	keywords = {Chromatography: High Pressure Liquid, Cluster Analysis, Flavonoids, Lamiaceae, Phylogeny, Plants: Medicinal, Saponins},
	author = {Xue-hui Zhang and Jian-min Chen}
}
@article {Shao:2006p8006,
	title = {[RAPD analysis of Scutellaria baicalensis from different germplasms]},
	journal = {Zhongguo Zhong Yao Za Zhi},
	volume = {31},
	number = {6},
	year = {2006},
	month = {Mar},
	pages = {452{\textendash}5},
	abstract = {OBJECTIVE: To provide reference for breeding Scutellaria baicalensis from different germplasms,and to explore their genetic diversities and different strains. METHOD: The RAPD method was applied to the study on S. baicalensis from different germplasms by use of 14 random primers about 10 bp, and SAPD-analysis of 34 S. baicalensis from different germplasms was carried out by using the method of withingroups-linkage in SPSS 10.0. RESULTS: 14 among 115 primers were selected to amplify about 165 segments of DNA. Among them, 132 segments of DNA, 80.0\% of the total, can represent genetic of diversities of S. baicalensis. According to RAPD analysis, 34 germplasms of S. baicalensis were classified into A, B, C and D categories. CONCLUSION: S. baicalensis from different germplasms shows abundant genetic diversities. Germplasms from Shandong like Mengyin 3, Menyin 2 and Pingyi have close distance (0.315) in genetic background, which can be chosen for breeding of cultivated. Though genetic characters are similar in the morphology, the geological distribution of S. baicalensis and its morphology have not certain correlation. The complex genetic background of S. baicalensis indicate that the work of the selective breeding and management for breeding of S. baicalensis have to be strengthened.},
	keywords = {Breeding, Cluster Analysis, DNA Primers, DNA: Plant, Ecosystem, Genetic Variation, Phylogeny, Plant Leaves, Plants: Medicinal, Random Amplified Polymorphic DNA Technique, Scutellaria baicalensis},
	author = {Ai-Juan Shao and Xin Li and Lu-Qi Huang and Shu-Fang Lin and Jun Chen}
}
