@article {PassinhoSoares:2006p7996,
	title = {Authentication of medicinal plant botanical identity by amplified fragmented length polymorphism dominant DNA marker: inferences from the Plectranthus genus},
	journal = {Planta Med},
	volume = {72},
	number = {10},
	year = {2006},
	month = {Aug},
	pages = {929{\textendash}31},
	abstract = {In Brazil, Plectranthus species are known as "boldo" and have been used in popular medicine for analgesic and dyspeptic purposes. Plectranthus need to be well identified in order to be used as commercially genuine medicinal plants. Here we describe AFLP DNA patterns able to distinguish among different Pectranthus species. The genetic variability of P. grandis Cramer, P. barbatus Andr. and P. ornatus Codd was analyzed with two sets of AFLP primers allowing detection of 241 loci. A total of 22 monomorphic loci were identified in P. barbatus, 15 in P. grandis and 30 in P. ornatus. Among these, 5 loci were informative and species-specific to P. barbatus, 3 to P. grandis and 2 loci were unique to P. ornatus. The AFLP pattern analyzed by different clustering methods assembled individuals according to their species. So far, AFLP represents a genuine and strong method to certify medicinal plant materials.},
	keywords = {DNA: Plant, Genetic Markers, Phylogeny, Plants: Medicinal, Plectranthus, Polymerase Chain Reaction, Polymorphism: Genetic, Species Specificity},
	doi = {10.1055/s-2006-946673},
	author = {Helna Passinho-Soares and Durvalina Felix and Maria Auxiliadora Kaplan and Marcia Margis-Pinheiro and Rog{\'e}rio Margis}
}
@article {Meimberg:2006p7985,
	title = {Molecular evidence for adaptive radiation of Micromeria Benth. (Lamiaceae) on the Canary Islands as inferred from chloroplast and nuclear DNA sequences and ISSR fingerprint data},
	journal = {Molecular Phylogenetics and Evolution},
	volume = {41},
	number = {3},
	year = {2006},
	month = {Dec},
	pages = {566{\textendash}78},
	abstract = {The Canary Islands have been a focus for phylogeographic studies on the colonization and diversification of endemic angiosperm taxa. Based on phylogeographic patterns, both inter island colonization and adaptive radiation seem to be the driving forces for speciation in most taxa. Here, we investigated the diversification of Micromeria on the Canary Islands and Madeira at the inter- and infraspecific level using inter simple sequence repeat PCR (ISSR), the trnK-Intron and the trnT-trnL-spacer of the cpDNA and a low copy nuclear gene. The genus Micromeria (Lamiaceae, Mentheae) includes 16 species and 13 subspecies in Macaronesia. Most taxa are restricted endemics, or grow in similar ecological conditions on two islands. An exception is M. varia, a widespread species inhabits the lowland scrub on each island of the archipelago and could represent an ancestral taxon from which radiation started on the different islands. Our analyses support a split between the "eastern" islands Fuerteventura, Lanzarote and Gran Canaria and the "western" islands Tenerife, La Palma and El Hierro. The colonization of Madeira started from the western Islands, probably from Tenerife as indicated by the sequence data. We identified two lineages of Micromeria on Gomera but all other islands appear to be colonized by a single lineage, supporting adaptive radiation as the major evolutionary force for the diversification of Micromeria. We also discuss the possible role of gene flow between lineages of different Micromeria species on one island after multiple colonizations.},
	keywords = {Adaptation: Biological, Atlantic Islands, Dna Fingerprinting, DNA Primers, DNA: Chloroplast, Genetic Variation, Geranyltranstransferase, Introns, Lamiaceae, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, Sequence Analysis: DNA},
	doi = {10.1016/j.ympev.2006.05.037},
	author = {Harald Meimberg and Tilmann Abele and Christian Br{\"a}uchler and John K McKay and Pedro L P{\'e}rez de Paz and G{\"u}nther Heubl}
}
@article {Berger:2006p7995,
	title = {Rosmarinic acid synthase is a new member of the superfamily of BAHD acyltransferases},
	journal = {Planta},
	volume = {224},
	number = {6},
	year = {2006},
	month = {Nov},
	pages = {1503{\textendash}10},
	abstract = {Purification of rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase) from suspension cells of Coleus blumei Benth. (Lamiaceae) by fractionated ammonium sulphate precipitation, hydrophobic interaction chromatography and two affinity chromatography steps led to the identification of peptide sequences, which enabled a PCR-based approach to isolate the full-length cDNA encoding this enzyme. The open reading frame of the cDNA had a length of 1290 base pairs encoding a protein of 430 amino acid residues with a molecular mass of 47,932 Da with typical characteristics of an acyltransferase of the BAHD superfamily. The cDNA was heterologously expressed in Escherichia coli. The enzyme displayed the activity of rosmarinic acid synthase using 4-coumaroyl- and caffeoyl-coenzyme A and 4-hydroxyphenyllactate as well as 3.4-dihydroxyphenyllactate as substrates. Shikimic acid and quinic acid were not able to serve as hydroxycinnamoyl acceptors. This therefore is the first report of the cDNA-cloning of a rosmarinic acid synthase.},
	keywords = {Acyltransferases, Amino Acid Sequence, Base Sequence, DNA: Complementary, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction},
	doi = {10.1007/s00425-006-0393-y},
	author = {Anja Berger and Juliane Meinhard and Maike Petersen}
}
@article {Kochieva:2006p7998,
	title = {[RAPD and ISSR analyses of species and populations of the genus Stachys]},
	journal = {Genetika},
	volume = {42},
	number = {7},
	year = {2006},
	month = {Jul},
	pages = {887{\textendash}92},
	abstract = {Molecular analysis of the genome was performed for 14 species of the genus Stachys. RAPD and ISSR analyses of the Stachys genome revealed 574 polymorphic fragments, including genus- and species-specific markers. Based on the patterns, UPGMA and the Jacquard coefficient were used to estimate the genetic distances between Stachys species and populations and to construct dendrograms reflecting the phylogenetic relationships among the Stachys species. Molecular analysis of the Stachys genome refined the phylogenetic positions of some species and revealed synonymous species.},
	keywords = {Chromosome Mapping, Chromosomes: Plant, Cluster Analysis, Genetic Markers, Genome: Plant, Phylogeny, Polymerase Chain Reaction, Polymorphism: Genetic, Random Amplified Polymorphic DNA Technique, Stachys},
	author = {E Z Kochieva and N N Ryzhova and M P Legkobit and N V Khadeeva}
}
