@article {Trusty:2005p8008,
	title = {Molecular phylogenetics of the Macaronesian-endemic genus Bystropogon (Lamiaceae): palaeo-islands, ecological shifts and interisland colonizations},
	journal = {Molecular ecology},
	volume = {14},
	number = {4},
	year = {2005},
	month = {Apr},
	pages = {1177{\textendash}89},
	abstract = {Abstract A molecular phylogenetic study of Bystropogon L{\textquoteright}Her. (Lamiaceae) is presented. We performed a cladistic analysis of nucleotide sequences of the internal transcribed spacers (ITS), of the nuclear ribosomal DNA, and of the trnL gene and trnL-trnF intergenic spacer of the chloroplast DNA. Bystropogon odoratissimus is the only species endemic to the Canary Islands that occurs in the three palaeo-islands of Tenerife. This species is not part of an early diverging lineage of Bystropogon and we suggest that it has a recent origin. This phylogenetic pattern is followed by most of the species endemic to the palaeo-islands of Tenerife. The two sections currently recognized in Bystropogon form two monophyletic groups. Taxa belonging to the section Bystropogon clade show interisland colonization limited to the Canary Islands with ecological shifts among three ecological zones. Taxa from the section Canariense clade show interisland colonization both within the Canary Islands and between the Canary Islands and Madeira. Speciation events within this clade are mostly limited to the laurel forest. The genus has followed a colonization route from the Canaries towards Madeira. This route has also been followed by at least five other plant genera with species endemic to Macaronesia. Major incongruences were found between the current infrasectional classification and the molecular phylogeny, because the varieties of Bystropogon origanifolius and Bystropogon canariensis do not form two monophyletic groups. The widespread B. origanifolius appears as progenitor of the other species in section Bystropogon with a more restricted distribution.},
	keywords = {DNA: Chloroplast, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Geography, Lamiaceae, Phylogeny, Sequence Analysis: DNA},
	doi = {10.1111/j.1365-294X.2005.02487.x},
	author = {Jennifer L Trusty and Richard G Olmstead and Arnoldo Santos-Guerra and Susana S{\'a}-Fontinha and Javier Francisco-Ortega}
}
@article {Wang:2007p7983,
	title = {[Analysis of ITS sequences of some medicinal plants and their related species in Salvia]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {12},
	year = {2007},
	month = {Dec},
	pages = {1309{\textendash}13},
	abstract = {Molecular systematic techniques were applied to reveal the genetic diversity of medicinal plants and their related species in Salvia. The internal transcribed spacer (ITS) as well as 5.8S rDNA sequences of 27 samples of Salvia were amplified using PCR method and sequenced. Mega 3.1 was used to analyze the genetic diversity within genus. The complete sequences of ITS plus 5.8S rDNA are about 612-617 bp. A phylogenetic tree generated by Neighbor-Joining method partly supported the morphological classification within Salvia, but incompatible results were also obtained in the treatment of phylogenetic positions of some species such as Salvia trijuga, Salvia flava var. flava and Salvia flava var. megalentha. The ITS regions of present Salria species showed considerable variation between subgenera in contrast with the conservative 5.8S rDNA sequences. The native Salvia species might have a different origin from the foreign species. The phylogenetic positions of subgenera and sections inferred by ITS analysis were comparable with that of traditional classification, while the phylogeny within sections is still doubtful due to limited information in ITS sequence and need to be further proved by other evidence. ITS analysis in this study supports the rationality of using species from Drymosphace section as substitute drug resources of Dan shen, but also reveals significant genetic differences between high mountain Dan shen species such as Salvia przewalskii with traditional Dan shen origins.},
	keywords = {Base Sequence, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genetic Variation, Phylogeny, Plants: Medicinal, Polymerase Chain Reaction, RNA: Ribosomal: 5.8S, Salvia, Sequence Alignment, Sequence Analysis: DNA},
	author = {Ying Wang and Da-hui Li and Ying-tao Zhang}
}
@article {Zhang:2007p7990,
	title = {[Correlation between ITS genotype and geographical distribution of Pogostemon cablin]},
	journal = {Yao Xue Xue Bao},
	volume = {42},
	number = {1},
	year = {2007},
	month = {Jan},
	pages = {93{\textendash}7},
	abstract = {To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.},
	keywords = {Base Sequence, Biodiversity, China, Cluster Analysis, DNA: Plant, DNA: Ribosomal, DNA: Ribosomal Spacer, Genotype, Geography, Lamiaceae, Molecular Sequence Data, Phylogeny, Plant Leaves, Plants: Medicinal, Sequence Analysis: DNA},
	author = {Ying Zhang and Yao Chen and Jin-Chao Zhang and Meng-Su Yang and Hui Cao and Pei-Gen Xiao}
}
@article {Yang:2006p8002,
	title = {[Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species]},
	journal = {Nan Fang Yi Ke Da Xue Xue Bao},
	volume = {26},
	number = {11},
	year = {2006},
	month = {Nov},
	pages = {1593{\textendash}5},
	abstract = {OBJECTIVE: To analyze the nuclear ribosome DNA (nrDNA) internal transcribed spacer (ITS) sequences of 4 Leonurus species, and the possibility of using them for molecular authentication of the crude drugs from the genus. METHODS: The nrDNA ITS sequence (including ITS1, 5.8S rDNA, ITS2, and partial 18S rDNA and 26S rDNA) of L. japonicus and its 3 adulterant species were amplified and sequenced, and CLUSTRAL X and MEGA software was employed for analysis. RESULTS: The variation of ITS1 and ITS2 between L. japonicus and its adulterant species ranged between 7.2\% and 18.8\% and between 14.2\% and 27\%, respectively. The phylogenic tree derived from the dendrograms based on the ITS sequence data contained some discrepancy from the traditional classification. CONCLUSION: The nrDNA ITS sequences can be used potentially as efficient markers for identification of L. japonicus and its adulterants, and further study is needed for studying the phylogeny of Leonurus.},
	keywords = {DNA: Plant, DNA: Ribosomal Spacer, Leonurus, Molecular Sequence Data, Phylogeny, Sequence Analysis: DNA, Species Specificity},
	author = {Zhi-Ye Yang and Zhi Chao and Ke-Ke Huo and Bing-Yi Wu and Sheng-li Pan}
}
