@article {Hosoi:2004p8023,
	title = {cDNA isolation and functional expression of myrcene synthase from Perilla frutescens},
	journal = {Biol Pharm Bull},
	volume = {27},
	number = {12},
	year = {2004},
	month = {Dec},
	pages = {1979{\textendash}85},
	abstract = {cDNA cloning of a monoterpene synthase from Perilla frutescens whose steam-distilled oil contains 92.9\% perillaketone, was performed by the PCR method using primers designed based on limonene synthase. The full-length nucleotide sequence of this cDNA consisted of 1978 bp including a 1827-bp translational region encoding a deduced protein of 608 amino acids, which was similar to that of limonene synthase from P. frutescens (85\% identity). Functional expression of this clone in Escherichia coli yielded an active monoterpene synthase enzyme, which converted geranyl diphosphate into 53.8\% myrcene, 20.9\% sabinene, 19.8\% linalool and 5.5\% limonene. As for the extraction of reaction products, we performed SPME (solid phase micro extraction) as well as conventional solvent extraction, and compared these two extraction methods.},
	keywords = {Amino Acid Sequence, DNA: Complementary, Gene Expression Regulation: Enzymologic, Intramolecular Lyases, Molecular Sequence Data, Perilla frutescens, Phylogeny},
	author = {Madoka Hosoi and Michiho Ito and Toru Yagura and Robert Phillip Adams and Gisho Honda}
}
