In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts

Publication Type:Journal Article
Year of Publication:2002
Authors:Dzhambazov, B, Daskalova, S, Monteva, A, Popov, N
Journal:Biol Pharm Bull
Volume:25
Pagination:499–504
Date Published:Jan
Keywords:Antitumour Activity, Chinensis, Clinopodium Vulgare L., Hela-Cells, Induced Phospholipid-Metabolism, Inhibitory Activity, Oleanane-Triterpene Saponins, Plant, Plant Extract, Steroidal Saponins, Ursolic Acid
Abstract:

Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 mug/ml it reduced the cell survival to 1.0, 5.6 and 6.6%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50% (IC50 values) were calculated to be 20, 10 and 17.8 mug/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible.

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