01789nas a2200229   4500008004100000245017500041210006900216260000800285300001400293490000700307520099500314653002001309653004401329653001201373653001001385653001301395100001601408700001701424700001501441700001601456856008701472       2007                        eng d00aPreparative isolation and purification of saponin and flavone glycoside compounds from Clinopodium chinensis (Benth) O. Kuntze by high-speed countercurrent chromatography0 aPreparative isolation and purification of saponin and flavone gl  cJan  a521–5320 v303 aA preparative high-speed countercurrent chromatography (HSCCC) method for isolation and purification of didymin, nairutin, and clinopodiside A from Clinopodium chinensis (Benth) O. Kuntze (Duanxueliu in Chinese) was successfully established. The separation was performed in two steps with two different kinds of solvent systems. In the first step, ethyl acetate-1-butanol-water (5:0.8:5, v/v) was used as the two-phase solvent system; nairutin was purified, and didymin and clinopodiside A were eluted together. In the second step, ethyl acetate-methanol-water (5:1:5, v/v) was used as the two-phase solvent system; didymin and clinopodiside A were separated and purified. After two-step separation, 15.2 mg of nairutin, 39.1 mg of clinopodiside A, and 20.6 mg of didymin were obtained from 100 mg of crude extract with purities of 96.5%, 98.4%, and 99.1%, respectively, as determined by HPLC analysis. The chemical structures of the three components were confirmed by H-1 NMR and C-13 NMR.10aClinopodiside A10aClinopodium Chinensis (Benth) O. Kuntze10aDidymin10aHsccc10aNairutin1 aLiu, Renmin1 aKong, Lingyi1 aLi, Aifeng1 aSun, Ailing  uhttp://www.informaworld.com/smpp/content db=all?content=10.1080/10826070601093846