02470nas a2200301   4500008004100000245009300041210006900134260000800203300001400211490000700225520151100232653001801743653001501761653001901776653002601795653002201821653001401843653002201857653003001879653002501909653001101934653002301945653002701968100001501995700001502010700002002025856012302045       2007                        eng d00a[Analysis of ITS sequences of some medicinal plants and their related species in Salvia]0 aAnalysis of ITS sequences of some medicinal plants and their rel  cDec  a1309–130 v423 aMolecular systematic techniques were applied to reveal the genetic diversity of medicinal plants and their related species in Salvia. The internal transcribed spacer (ITS) as well as 5.8S rDNA sequences of 27 samples of Salvia were amplified using PCR method and sequenced. Mega 3.1 was used to analyze the genetic diversity within genus. The complete sequences of ITS plus 5.8S rDNA are about 612-617 bp. A phylogenetic tree generated by Neighbor-Joining method partly supported the morphological classification within Salvia, but incompatible results were also obtained in the treatment of phylogenetic positions of some species such as Salvia trijuga, Salvia flava var. flava and Salvia flava var. megalentha. The ITS regions of present Salria species showed considerable variation between subgenera in contrast with the conservative 5.8S rDNA sequences. The native Salvia species might have a different origin from the foreign species. The phylogenetic positions of subgenera and sections inferred by ITS analysis were comparable with that of traditional classification, while the phylogeny within sections is still doubtful due to limited information in ITS sequence and need to be further proved by other evidence. ITS analysis in this study supports the rationality of using species from Drymosphace section as substitute drug resources of Dan shen, but also reveals significant genetic differences between high mountain Dan shen species such as Salvia przewalskii with traditional Dan shen origins.10aBase Sequence10aDNA: Plant10aDNA: Ribosomal10aDNA: Ribosomal Spacer10aGenetic Variation10aPhylogeny10aPlants: Medicinal10aPolymerase Chain Reaction10aRNA: Ribosomal: 5.8S10aSalvia10aSequence Alignment10aSequence Analysis: DNA1 aWang, Ying1 aLi, Da-hui1 aZhang, Ying-tao  uhttps://lamiaceae.myspecies.info/content/analysis-its-sequences-some-medicinal-plants-and-their-related-species-salvia01879nas a2200241   4500008004100000245008900041210006900130260000800199300001400207490000800221520107600229653002101305653002401326653001801350653002301368653002801391653001401419653003001433100001701463700002201480700002001502856011501522       2006                        eng d00aRosmarinic acid synthase is a new member of the superfamily of BAHD acyltransferases0 aRosmarinic acid synthase is a new member of the superfamily of B  cNov  a1503–100 v2243 aPurification of rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase) from suspension cells of Coleus blumei Benth. (Lamiaceae) by fractionated ammonium sulphate precipitation, hydrophobic interaction chromatography and two affinity chromatography steps led to the identification of peptide sequences, which enabled a PCR-based approach to isolate the full-length cDNA encoding this enzyme. The open reading frame of the cDNA had a length of 1290 base pairs encoding a protein of 430 amino acid residues with a molecular mass of 47,932 Da with typical characteristics of an acyltransferase of the BAHD superfamily. The cDNA was heterologously expressed in Escherichia coli. The enzyme displayed the activity of rosmarinic acid synthase using 4-coumaroyl- and caffeoyl-coenzyme A and 4-hydroxyphenyllactate as well as 3.4-dihydroxyphenyllactate as substrates. Shikimic acid and quinic acid were not able to serve as hydroxycinnamoyl acceptors. This therefore is the first report of the cDNA-cloning of a rosmarinic acid synthase.10aAcyltransferases10aAmino Acid Sequence10aBase Sequence10aDNA: Complementary10aMolecular Sequence Data10aPhylogeny10aPolymerase Chain Reaction1 aBerger, Anja1 aMeinhard, Juliane1 aPetersen, Maike  uhttps://lamiaceae.myspecies.info/content/rosmarinic-acid-synthase-new-member-superfamily-bahd-acyltransferases