01879nas a2200241   4500008004100000245008900041210006900130260000800199300001400207490000800221520107600229653002101305653002401326653001801350653002301368653002801391653001401419653003001433100001701463700002201480700002001502856011501522       2006                        eng d00aRosmarinic acid synthase is a new member of the superfamily of BAHD acyltransferases0 aRosmarinic acid synthase is a new member of the superfamily of B  cNov  a1503–100 v2243 aPurification of rosmarinic acid synthase (hydroxycinnamoyl-CoA:hydroxyphenyllactate hydroxycinnamoyltransferase) from suspension cells of Coleus blumei Benth. (Lamiaceae) by fractionated ammonium sulphate precipitation, hydrophobic interaction chromatography and two affinity chromatography steps led to the identification of peptide sequences, which enabled a PCR-based approach to isolate the full-length cDNA encoding this enzyme. The open reading frame of the cDNA had a length of 1290 base pairs encoding a protein of 430 amino acid residues with a molecular mass of 47,932 Da with typical characteristics of an acyltransferase of the BAHD superfamily. The cDNA was heterologously expressed in Escherichia coli. The enzyme displayed the activity of rosmarinic acid synthase using 4-coumaroyl- and caffeoyl-coenzyme A and 4-hydroxyphenyllactate as well as 3.4-dihydroxyphenyllactate as substrates. Shikimic acid and quinic acid were not able to serve as hydroxycinnamoyl acceptors. This therefore is the first report of the cDNA-cloning of a rosmarinic acid synthase.10aAcyltransferases10aAmino Acid Sequence10aBase Sequence10aDNA: Complementary10aMolecular Sequence Data10aPhylogeny10aPolymerase Chain Reaction1 aBerger, Anja1 aMeinhard, Juliane1 aPetersen, Maike  uhttps://lamiaceae.myspecies.info/content/rosmarinic-acid-synthase-new-member-superfamily-bahd-acyltransferases