@article {Matsuno:2002p8029,
	title = {CYP98A6 from Lithospermum erythrorhizon encodes 4-coumaroyl-4{\textquoteright}-hydroxyphenyllactic acid 3-hydroxylase involved in rosmarinic acid biosynthesis},
	journal = {FEBS Lett},
	volume = {514},
	number = {2-3},
	year = {2002},
	month = {Mar},
	pages = {219{\textendash}24},
	abstract = {Rosmarinic acid is the dominant hydroxycinnamic acid ester accumulated in Boraginaceae and Lamiaceae plants. A cytochrome P450 cDNA was isolated by differential display from cultured cells of Lithospermum erythrorhizon, and the gene product was designated CYP98A6 based on the deduced amino acid sequence. After expression in yeast, the P450 was shown to catalyze the 3-hydroxylation of 4-coumaroyl-4{\textquoteright}-hydroxyphenyllactic acid, one of the final two steps leading to rosmarinic acid. The expression level of CYP98A6 is dramatically increased by addition of yeast extract or methyl jasmonate to L. erythrorhizon cells, and its expression pattern reflected the elicitor-induced change in rosmarinic acid production, indicating that CYP98A6 plays an important role in regulation of rosmarinic acid biosynthesis.},
	keywords = {Acetates, Cell Extracts, Cells: Cultured, Cinnamates, Cloning: Molecular, Cyclopentanes, Cytochrome P-450 Enzyme System, Depsides, DNA: Complementary, Gene Expression, Gene Expression Profiling, Lithospermum, Mixed Function Oxygenases, Molecular Sequence Data, Oxylipins, Phenylalanine Ammonia-Lyase, Phenylpropionates, Phylogeny, Plant Proteins, RNA: Messenger},
	author = {Michiyo Matsuno and Akito Nagatsu and Yukio Ogihara and Brian E Ellis and Hajime Mizukami}
}
@article {Kitada:2001p8039,
	title = {Differential expression of two cytochrome P450s involved in the biosynthesis of flavones and anthocyanins in chemo-varietal forms of Perilla frutescens},
	journal = {Plant Cell Physiol},
	volume = {42},
	number = {12},
	year = {2001},
	month = {Dec},
	pages = {1338{\textendash}44},
	abstract = {In Perilla frutescens, there are two varietal forms of anthocyanin accumulation, i.e. red and green forms. The cDNA clones encoding flavone synthase II (FSII) and flavonoid 3{\textquoteright}-hydroxylase (F3{\textquoteright}H), two cytochrome P450s that are involved in the biosynthesis of flavones and anthocyanins, were isolated from P. frutescens. The FSII cDNA encoded a 57.1 kDa protein designated as CYP93B6, and the F3{\textquoteright}H cDNA encoded 57.5 kDa protein designated as CYP75B4. Recombinant CYP93B6 expressed in yeast converted flavanones to flavones with K(m) values of 8.8-11.9 microM. Recombinant CYP75B4 catalyzed 3{\textquoteright}-hydroxylation of flavanones to the corresponding compounds with K(m) values of 18-20 microM. The CYP93B6 transcript accumulated to an equal level in leaves of both red and green forms of P. frutescens, in agreement with the accumulation pattern of flavones in the leaves. However, the CYP75B4 transcript was predominantly expressed in the red form of P. frutescens, and its expression was induced by light in conjunction with other transcripts of biosynthetic enzymes of anthocyanin. These results indicate that gene expression of a set of anthocyanin biosynthetic enzymes including F3{\textquoteright}H is regulated coordinately only in the red form of P. frutescens but not in the green form, whilst FSII gene expression is controlled in a similar manner in red and green forms of P. frutescens.},
	keywords = {Anthocyanins, Apigenin, Cytochrome P-450 Enzyme System, Flavonoids, Gene Expression Regulation: Enzymologic, Gene Expression Regulation: Plant, Kinetics, Lamiaceae, Luteolin, Mixed Function Oxygenases, Phylogeny, Plant Structures},
	author = {C Kitada and Z Gong and Y Tanaka and M Yamazaki and K Saito}
}
