@article {Rosch:2002p8031,
	title = {Chemotaxonomy of mints of genus Mentha by applying Raman spectroscopy},
	journal = {Biopolymers},
	volume = {67},
	number = {4-5},
	year = {2002},
	month = {Jan},
	pages = {358{\textendash}61},
	abstract = {The characterization of mints is often problematic because Mentha is a taxonomically complex genus. In order to provide a fast and easy characterization method, we use a combination of micro-Raman spectroscopy and hierarchical cluster analysis. A classification trial of different mint taxa is possible for one collection time. For spectra measured at different points during the growing season, a more sophisticated pretreatment of the data is necessary to receive good discrimination between the species, as well as between the subspecies and varieties of the mints.},
	keywords = {Algorithms, Cluster Analysis, Mentha, Phylogeny, Spectrum Analysis: Raman},
	doi = {10.1002/bip.10099},
	author = {P R{\"o}sch and W Kiefer and J Popp}
}
@article {Pedranzani:2002p1148,
	title = {Regeneration of entire Hedeoma multiflorum Bentham plants through in vitro cultivation},
	journal = {Phyton-Int J Exp Bot},
	year = {2002},
	month = {Jan},
	pages = {207{\textendash}212},
	abstract = {Hedeoma multiflorum Bentham is an aromatic species in danger of extinction in the hilly country of the provinces of San Luis and Cordoba, Argentina. Our aim was to regenerate and propagate it in vitro to get plantlets for future plans of conservation of genetic resources. Tissue disinfectants and in vitro culture media were tested. Whole plants were obtained, strong, vigorous and in excellent sanitary conditions which, after acclimatization, were apt for outdoor transplanting.},
	url = {http://apps.isiknowledge.com/InboundService.do?product=WOS\&action=retrieve\&SrcApp=Papers\&UT=000203005900028\&SID=2AFL5dnj5MCc8JPedL5\&SrcAuth=mekentosj\&mode=FullRecord\&customersID=mekentosj\&DestFail=http\%253A\%252F\%252Faccess.isiproducts.com\%252Fcustom_image},
	author = {H. E Pedranzani and A. M Quiroga and E Martinez and E Fernandez}
}
@article {Prather:2002p1092,
	title = {Monophyly and phylogeny of Monarda (Lamiaceae): Evidence from the internal transcribed spacer (ITS) region of nuclear ribosomal DNA},
	journal = {Systematic Botany},
	volume = {27},
	number = {1},
	year = {2002},
	month = {Jan},
	pages = {127{\textendash}137},
	abstract = {The phylogenetic relationships of the 16 species of Monarda (Lamiaceae) were investigated using sequences of the internal transcribed spacer regions of nuclear ribosomal DNA. Thymus and Mentha were used as outgroups, and Blephilia, Clinopodium, Conradina, Hesperozygis, Monardella, Pycnanthemum, and Ziziphora were included in the ingroup to test the monophyly of Monarda. Two parsimony searches were performed after removing redundant sequences from the analysis: one with indels scored as missing and a second with indels treated as binary characters, Both searches yielded congruent results, but the treatment of indels as binary characters resulted in considerably more resolution within Monarda. There was strong support for the monophyly of Monarda and a close relationship was found between Monarda, Blephilia, and Pycnanthemum. The molecular phylogeny was completely congruent with the infrageneric classification of the genus. Our results were consistent with hypotheses of hybridization between M. fistulosa and M. lindheimeri in Texas. Despite considerable morphological variation among many species, especially in floral characters, little molecular diversification was found in those same species groups. Intraspecific polymorphism in ITS sequence was found in over half the species examined, and may be attributable to ancestral polymorphism, hybridization, or detection of paralogous loci.},
	keywords = {Classification, Evolution, Gene Trees, Islands, Labiatae, Molecular Systematics, Most-Parsimonious Trees, Patterns, Sequence Data, Species Trees},
	url = {http://apps.isiknowledge.com/InboundService.do?product=WOS\&action=retrieve\&SrcApp=Papers\&UT=000174306000012\&SID=2AFL5dnj5MCc8JPedL5\&SrcAuth=mekentosj\&mode=FullRecord\&customersID=mekentosj\&DestFail=http\%253A\%252F\%252Faccess.isiproducts.com\%252Fcustom_image},
	author = {LA Prather and AK Monfils and AL Posto and RA Williams}
}
@article {Barber:2002p8028,
	title = {Origin of Macaronesian Sideritis L. (Lamioideae: Lamiaceae) inferred from nuclear and chloroplast sequence datasets},
	journal = {Molecular Phylogenetics and Evolution},
	volume = {23},
	number = {3},
	year = {2002},
	month = {Jun},
	pages = {293{\textendash}306},
	abstract = {Sideritis L. (Lamiaceae) comprises approximately 150 species of annuals and perennials distributed chiefly in the Mediterranean region. The majority of the species belong to the continental subgenus Sideritis which is divided into two perennial (Sideritis and Empedoclea) and two annual (Hesiodia and Burgsdorfia) sections. Twenty-three species are woody perennials endemic to the Macaronesian archipelagos of Madeira and the Canary Islands. In an effort to determine the continental origin of the insular group, we constructed independent phylogenies comprising sequence data from both chloroplast and nuclear markers. Sampling included 7 island taxa drawn from the Macaronesian subgenus Marrubiastrum and 25 continental taxa representing all four sections of subgenus Sideritis. Subgenus Marrubiastrum and the two continental perennial sections form well-supported monophyletic groups in both individual and combined analyses. The annual sections are not monophyletic in any analysis; further sampling of annual taxa is needed to resolve these relationships. All analyses identified Sideritis cossoniana, an annual species from Morocco, as the closest continental relative of the Macaronesian group. This contrasts with the hypothesis of earlier workers who suggested that the insular taxa were most closely related to eastern Mediterranean species of the genus. The phylogenies also demonstrate a distinct increase in woodiness among the Macaronesian species relative to their continental congeners, providing further support for the secondary nature of woodiness in island plants.},
	keywords = {Biological Evolution, Cell Nucleus, Chloroplasts, DNA: Intergenic, DNA: Ribosomal, Genetic Variation, Introns, Phylogeny, Portugal, Sideritis, Spain},
	url = {http://linkinghub.elsevier.com/retrieve/pii/S1055-7903(02)00018-0},
	author = {Janet C Barber and Javier Francisco-Ortega and Arnoldo Santos-Guerra and Kathryn G Turner and Robert K Jansen}
}
@article {Gang:2002p8027,
	title = {Characterization of phenylpropene O-methyltransferases from sweet basil: facile change of substrate specificity and convergent evolution within a plant O-methyltransferase family},
	journal = {Plant Cell},
	volume = {14},
	number = {2},
	year = {2002},
	month = {Feb},
	pages = {505{\textendash}19},
	abstract = {Some basil varieties are able to convert the phenylpropenes chavicol and eugenol to methylchavicol and methyleugenol, respectively. Chavicol O-methyltransferase (CVOMT) and eugenol O-methyltransferase (EOMT) cDNAs were isolated from the sweet basil variety EMX-1 using a biochemical genomics approach. These cDNAs encode proteins that are 90\% identical to each other and very similar to several isoflavone O-methyltransferases such as IOMT, which catalyzes the 4{\textquoteright}-O-methylation of 2,7,4{\textquoteright}-trihydroxyisoflavanone. On the other hand, CVOMT1 and EOMT1 are related only distantly to (iso)eugenol OMT from Clarkia breweri, indicating that the eugenol O-methylating enzymes in basil and C. breweri evolved independently. Transcripts for CVOMT1 and EOMT1 were highly expressed in the peltate glandular trichomes on the surface of the young basil leaves. The CVOMT1 and EOMT1 cDNAs were expressed in Escherichia coli, and active proteins were produced. CVOMT1 catalyzed the O-methylation of chavicol, and EOMT1 also catalyzed the O-methylation of chavicol with equal efficiency to that of CVOMT1, but it was much more efficient in O-methylating eugenol. Molecular modeling, based on the crystal structure of IOMT, suggested that a single amino acid difference was responsible for the difference in substrate discrimination between CVOMT1 and EOMT1. This prediction was confirmed by site-directed mutagenesis, in which the appropriate mutants of CVOMT1 (F260S) and EOMT1 (S261F) were produced that exhibited the opposite substrate preference relative to the respective native enzyme.},
	keywords = {Amino Acid Sequence, Anisoles, DNA: Complementary, Escherichia coli, Eugenol, Evolution: Molecular, Gene Expression, Gene Expression Regulation: Enzymologic, Gene Expression Regulation: Plant, Isoflavones, Methyltransferases, Molecular Sequence Data, Molecular Structure, Mutagenesis: Site-Directed, Mutation, Ocimum basilicum, Phylogeny, Recombinant Proteins, Sequence Homology: Amino Acid, Substrate Specificity},
	author = {David R Gang and Noa Lavid and Chloe Zubieta and Feng Chen and Till Beuerle and Efraim Lewinsohn and Joseph P Noel and Eran Pichersky}
}
@booklet {AbdelMogib:2002p1132,
	title = {Chemistry of the genus Plectranthus},
	howpublished = {Molecules},
	volume = {7},
	number = {2},
	year = {2002},
	month = {Jan},
	pages = {271{\textendash}301},
	abstract = {This review presents the phytochemical constituents of the genus Plectranthus reported up to 1999. Only a tetrameric derivative of caffeic acid was isolated from P. japonicus, but a group of long-chain alkylphenols, of possible taxonomic significance in the genus, was also isolated. As a genus of the subfamily Nepetoideae, Plectranthus is free from iridoid glycosides and rich in essential oil (i.e. > 0.5\% volatile oil on a dry weight basis). Diterpenoids are the more common secondary metabolites in Plectranthus. The majority of them are highly modified abietanoids. This seems to be similar to the pattern of diterpenoids observed for Salvia, but no clerodane diterpenoids were found in Plectranthus.},
	keywords = {Abietane Diterpenoids, Antimicrobial Abietane, Chemical-Composition, Coleus, Diterpenoids, Essential Oil, Hereroensis, Labiatae, Lamiaceae, Leaf-Gland Pigments, Phytochemical Constituents, Plants, Plectranthus, Southern Africa},
	url = {http://apps.isiknowledge.com/InboundService.do?product=WOS\&action=retrieve\&SrcApp=Papers\&UT=000174211500017\&SID=2AFL5dnj5MCc8JPedL5\&SrcAuth=mekentosj\&mode=FullRecord\&customersID=mekentosj\&DestFail=http\%253A\%252F\%252Faccess.isiproducts.com\%252Fcustom_image},
	author = {M Abdel-Mogib and HA Albar and SM Batterjee}
}
@article {Gobert:2002p2225,
	title = {Hybridization in the Section Mentha (Lamiaceae) Inferred from AFLP Markers},
	journal = {American Journal of Botany},
	volume = {89},
	number = {12},
	year = {2002},
	month = {Dec},
	pages = {2017{\textendash}2023},
	abstract = {The amplified fragment length polymorphism (AFLP) method was used to evaluate genetic diversity and to assess genetic relationships within the section Mentha in order to clarify the taxonomy of several interspecific mint hybrids with molecular markers. To this end, genetic diversity of 62 Mentha accessions from different geographic origins, representing five species and three hybrids, was assessed. Three EcoRI/MseI AFLP primer combinations generated an average of 40 AFLP markers per primer combination, ranging in size from 50 to 500 base pairs (bp). The percentage of markers polymorphic ranged from 50\% to 60\% across all accessions studied. According to phenetic and cladistic analysis, the 62 mint accessions were grouped into two major clusters. Principal coordinates analysis separated species into well-defined groups, and clear relationships between species and hybrids could be described. Our AFLP analysis supports taxonomic classification established among Mentha species by conventional (morphological, cytological, and chemical) methods. It allows the assessment of phenetic relationships between species and the hybrids M. spicata and M. x piperita, largely cultivated all over the world for their menthol source, and provides new insights into the subdivision of M. spicata, based for the first time on molecular markers.},
	keywords = {Genetic Diversity},
	url = {http://www.jstor.org/stable/4122756},
	author = {V Gobert and S Moja and M Colson and P Taberlet}
}
@article {Dzhambazov:2002p1174,
	title = {In vitro screening for antitumour activity of Clinopodium vulgare L. (Lamiaceae) extracts},
	journal = {Biol Pharm Bull},
	volume = {25},
	number = {4},
	year = {2002},
	month = {Jan},
	pages = {499{\textendash}504},
	abstract = {Aqueous extract of Clinopodium vulgare L. showed strong antitumour activity when tested in vitro on A2058 (human metastatic melanoma), HEp-2 (epidermoid carcinoma, larynx, human) and L5178Y (mouse lymphoma) cell lines-6 h after treatment disintegration of the nuclei and cell lysis started. Applied at a concentration of 80 mug/ml it reduced the cell survival to 1.0, 5.6 and 6.6\%, respectively. The concentrations of aqueous extract inhibiting the growth of A2058, HEp-2 and L5178Y cells by 50\% (IC50 values) were calculated to be 20, 10 and 17.8 mug/ml respectively. Two groups of active substances were detected: the first one, probably combining glycosides, influenced adhesion, while the second one caused massive cell vacuolisation. The chloroform extract, which contained ursolic acid and gentriacontan had also cytotoxic, however a little bit weaker effect. All changes observed were irreversible.},
	keywords = {Antitumour Activity, Chinensis, Clinopodium Vulgare L., Hela-Cells, Induced Phospholipid-Metabolism, Inhibitory Activity, Oleanane-Triterpene Saponins, Plant, Plant Extract, Steroidal Saponins, Ursolic Acid},
	url = {http://apps.isiknowledge.com/InboundService.do?product=WOS\&action=retrieve\&SrcApp=Papers\&UT=000174690200019\&SID=2AFL5dnj5MCc8JPedL5\&SrcAuth=mekentosj\&mode=FullRecord\&customersID=mekentosj\&DestFail=http\%253A\%252F\%252Faccess.isiproducts.com\%252Fcustom_image},
	author = {B Dzhambazov and S Daskalova and A Monteva and N Popov}
}
@article {Gobert:2002p2221,
	title = {Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers},
	journal = {Am J Bot},
	volume = {89},
	number = {12},
	year = {2002},
	month = {Jan},
	pages = {2017{\textendash}2023},
	abstract = {The amplified fragment length polymorphism (AFLP) method was used to evaluate genetic diversity and to assess genetic relationships within the section Mentha in order to clarify the taxonomy of several interspecific mint hybrids with molecular markers. To this end, genetic diversity of 62 Mentha accessions from different geographic origins, representing five species and three hybrids, was assessed. Three EcoRI/MseI AFLP primer combinations generated an average of 40 AFLP markers per primer combination, ranging in size from 50 to 500 base pairs (bp). The percentage of markers polymorphic ranged from 50\% to 60\% across all accessions studied. According to phenetic and cladistic analysis, the 62 mint accessions were grouped into two major clusters. Principal coordinates analysis separated species into well-defined groups, and clear relationships between species and hybrids could be described. Our AFLP analysis supports taxonomic classification established among Mentha species by conventional (morphological, cytological, and chemical) methods. It allows the assessment of phenetic relationships between species and the hybrids M. spicata and M. X piperita, largely cultivated all over the world for their menthol source, and provides new insights into the subdivision of M. spicata, based for the first time on molecular markers.},
	keywords = {Aflp, Dna Fingerprinting, Genetic Diversity, Hybridization, Lamiaceae, Lines, Mentha, Polyploid, Rapd, Rflp},
	author = {V Gobert and S Moja and M Colson and P Taberlet}
}
@article {Matsuno:2002p8029,
	title = {CYP98A6 from Lithospermum erythrorhizon encodes 4-coumaroyl-4{\textquoteright}-hydroxyphenyllactic acid 3-hydroxylase involved in rosmarinic acid biosynthesis},
	journal = {FEBS Lett},
	volume = {514},
	number = {2-3},
	year = {2002},
	month = {Mar},
	pages = {219{\textendash}24},
	abstract = {Rosmarinic acid is the dominant hydroxycinnamic acid ester accumulated in Boraginaceae and Lamiaceae plants. A cytochrome P450 cDNA was isolated by differential display from cultured cells of Lithospermum erythrorhizon, and the gene product was designated CYP98A6 based on the deduced amino acid sequence. After expression in yeast, the P450 was shown to catalyze the 3-hydroxylation of 4-coumaroyl-4{\textquoteright}-hydroxyphenyllactic acid, one of the final two steps leading to rosmarinic acid. The expression level of CYP98A6 is dramatically increased by addition of yeast extract or methyl jasmonate to L. erythrorhizon cells, and its expression pattern reflected the elicitor-induced change in rosmarinic acid production, indicating that CYP98A6 plays an important role in regulation of rosmarinic acid biosynthesis.},
	keywords = {Acetates, Cell Extracts, Cells: Cultured, Cinnamates, Cloning: Molecular, Cyclopentanes, Cytochrome P-450 Enzyme System, Depsides, DNA: Complementary, Gene Expression, Gene Expression Profiling, Lithospermum, Mixed Function Oxygenases, Molecular Sequence Data, Oxylipins, Phenylalanine Ammonia-Lyase, Phenylpropionates, Phylogeny, Plant Proteins, RNA: Messenger},
	author = {Michiyo Matsuno and Akito Nagatsu and Yukio Ogihara and Brian E Ellis and Hajime Mizukami}
}
