@article {Kim:2004p8024,
	title = {Paenibacillus elgii sp. nov., with broad antimicrobial activity},
	journal = {Int J Syst Evol Microbiol},
	volume = {54},
	number = {Pt 6},
	year = {2004},
	month = {Nov},
	pages = {2031{\textendash}5},
	abstract = {Two novel spore-forming bacteria with broad antimicrobial activity were isolated from roots of Perilla frutescens. The isolates, SD17(T) and SD18, were facultatively anaerobic and showed variable Gram reaction. Growth was observed between 20 and 45 degrees C. DNA G+C content of SD17(T) was 51.7 mol\%, and the major fatty acid was anteiso-C(15 : 0) (54.1 \%). 16S rRNA gene sequence similarity of SD17(T) ranged from 98.6 to 91.3 \% with other Paenibacillus species. The phylogenetic tree showed that isolate SD17(T) formed a significant monophyletic clade with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T). DNA-DNA relatedness values for strain SD17(T) with Paenibacillus koreensis KCTC 2393(T) and Paenibacillus ehimensis IFO 15659(T) were 17.4 and 19.8 \%, respectively. These isolates thus merit species status within Paenibacillus, for which the name Paenibacillus elgii sp. nov. is proposed. The type strain is SD17(T) (=KCTC 10016BP(T)=NBRC 100335(T)).},
	keywords = {Anaerobiosis, Anti-Bacterial Agents, Bacterial Typing Techniques, Base Composition, DNA: Bacterial, DNA: Ribosomal, Fatty Acids, Genes: rRNA, Gram-Positive Endospore-Forming Bacteria, Molecular Sequence Data, Perilla frutescens, Phylogeny, Plant Roots, RNA: Bacterial, RNA: Ribosomal: 16S, Sequence Analysis: DNA, Temperature},
	doi = {10.1099/ijs.0.02414-0},
	author = {Dal-Soo Kim and Cheol-Yong Bae and Jae-Jin Jeon and Sam-Jae Chun and Hyun Woo Oh and Soon Gyu Hong and Keun-Sik Baek and Eun Young Moon and Kyung Sook Bae}
}
@article {Hosoi:2004p8023,
	title = {cDNA isolation and functional expression of myrcene synthase from Perilla frutescens},
	journal = {Biol Pharm Bull},
	volume = {27},
	number = {12},
	year = {2004},
	month = {Dec},
	pages = {1979{\textendash}85},
	abstract = {cDNA cloning of a monoterpene synthase from Perilla frutescens whose steam-distilled oil contains 92.9\% perillaketone, was performed by the PCR method using primers designed based on limonene synthase. The full-length nucleotide sequence of this cDNA consisted of 1978 bp including a 1827-bp translational region encoding a deduced protein of 608 amino acids, which was similar to that of limonene synthase from P. frutescens (85\% identity). Functional expression of this clone in Escherichia coli yielded an active monoterpene synthase enzyme, which converted geranyl diphosphate into 53.8\% myrcene, 20.9\% sabinene, 19.8\% linalool and 5.5\% limonene. As for the extraction of reaction products, we performed SPME (solid phase micro extraction) as well as conventional solvent extraction, and compared these two extraction methods.},
	keywords = {Amino Acid Sequence, DNA: Complementary, Gene Expression Regulation: Enzymologic, Intramolecular Lyases, Molecular Sequence Data, Perilla frutescens, Phylogeny},
	author = {Madoka Hosoi and Michiho Ito and Toru Yagura and Robert Phillip Adams and Gisho Honda}
}
