@article {PassinhoSoares:2006p7996,
	title = {Authentication of medicinal plant botanical identity by amplified fragmented length polymorphism dominant DNA marker: inferences from the Plectranthus genus},
	journal = {Planta Med},
	volume = {72},
	number = {10},
	year = {2006},
	month = {Aug},
	pages = {929{\textendash}31},
	abstract = {In Brazil, Plectranthus species are known as "boldo" and have been used in popular medicine for analgesic and dyspeptic purposes. Plectranthus need to be well identified in order to be used as commercially genuine medicinal plants. Here we describe AFLP DNA patterns able to distinguish among different Pectranthus species. The genetic variability of P. grandis Cramer, P. barbatus Andr. and P. ornatus Codd was analyzed with two sets of AFLP primers allowing detection of 241 loci. A total of 22 monomorphic loci were identified in P. barbatus, 15 in P. grandis and 30 in P. ornatus. Among these, 5 loci were informative and species-specific to P. barbatus, 3 to P. grandis and 2 loci were unique to P. ornatus. The AFLP pattern analyzed by different clustering methods assembled individuals according to their species. So far, AFLP represents a genuine and strong method to certify medicinal plant materials.},
	keywords = {DNA: Plant, Genetic Markers, Phylogeny, Plants: Medicinal, Plectranthus, Polymerase Chain Reaction, Polymorphism: Genetic, Species Specificity},
	doi = {10.1055/s-2006-946673},
	author = {Helna Passinho-Soares and Durvalina Felix and Maria Auxiliadora Kaplan and Marcia Margis-Pinheiro and Rog{\'e}rio Margis}
}
@article {Yang:2006p8002,
	title = {[Nuclear ribosomal DNA internal transcribed spacer 1 sequences of 4 Leonurus species]},
	journal = {Nan Fang Yi Ke Da Xue Xue Bao},
	volume = {26},
	number = {11},
	year = {2006},
	month = {Nov},
	pages = {1593{\textendash}5},
	abstract = {OBJECTIVE: To analyze the nuclear ribosome DNA (nrDNA) internal transcribed spacer (ITS) sequences of 4 Leonurus species, and the possibility of using them for molecular authentication of the crude drugs from the genus. METHODS: The nrDNA ITS sequence (including ITS1, 5.8S rDNA, ITS2, and partial 18S rDNA and 26S rDNA) of L. japonicus and its 3 adulterant species were amplified and sequenced, and CLUSTRAL X and MEGA software was employed for analysis. RESULTS: The variation of ITS1 and ITS2 between L. japonicus and its adulterant species ranged between 7.2\% and 18.8\% and between 14.2\% and 27\%, respectively. The phylogenic tree derived from the dendrograms based on the ITS sequence data contained some discrepancy from the traditional classification. CONCLUSION: The nrDNA ITS sequences can be used potentially as efficient markers for identification of L. japonicus and its adulterants, and further study is needed for studying the phylogeny of Leonurus.},
	keywords = {DNA: Plant, DNA: Ribosomal Spacer, Leonurus, Molecular Sequence Data, Phylogeny, Sequence Analysis: DNA, Species Specificity},
	author = {Zhi-Ye Yang and Zhi Chao and Ke-Ke Huo and Bing-Yi Wu and Sheng-li Pan}
}
